Anti-MAP2抗体(ab75713)

概述

  • 产品名称Anti-MAP2抗体
    参阅全部 MAP2 一抗
  • 描述
    鸡多克隆抗体to MAP2
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Two synthetic peptidescorresponding to different regions of the MAP2 gene product, but shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences conjugated to KLH.

  • 阳性对照
    • Cultured neurons from an e13.5 mouse brain.
  • 常规说明


    Two different affinity purified anti peptide antibodies were combined to make this product. The concentrations of both of these antibodies was 100 µg/ml, making the total antibody concentration 200 µg/ml.

性能

应用

Our Abpromise guarantee covers the use of ab75713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/5000. Predicted molecular weight: 200 kDa.
IHC-P 1/1000 - 1/2000.
ICC/IF 1/1000 - 1/2000.

靶标

Anti-MAP2 antibody 图像

  • Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP.

  • Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP, Blue - DAPI nuclear stain.

Anti-MAP2 antibody (ab75713)参考文献

ab75713 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and thank you for submitting an abreview. The abreview program is only for testing primary antibodies in untested species/assays. Unfortunately it does not include secondary antibodies. However, it looks as the seconday anti...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Acetone
Permeabilization Yes - 0.3% Triton X-100/PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Julie Nigro

Verified customer

提交于 Dec 28 2011

Thank you for submitting an Abreview of ab56164. As you may have noticed, your review has now been published on our website. We appreciate the time and effort that you have put into this and would like to express our gratitude. If there is anything tha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"