概述

  • 产品名称
  • 描述
    鸡多克隆抗体to MAP2
  • 经测试应用
    适用于: ELISA, IHC-Fr, IHC-FoFr, IHC-P, WB, ICC/IF, IHC (PFA fixed)more details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Cow, Dog, Human, Cynomolgus monkey, Common marmoset, Aplysia
  • 免疫原

    Full length native protein (purified) corresponding to Cow MAP2.

  • 阳性对照
    • ICC: cultured rat cortical neurons

性能

应用

Our Abpromise guarantee covers the use of ab5392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
IHC-Fr Use at an assay dependent dilution.
IHC-FoFr 1/500. PubMed: 18684835
IHC-P Use at an assay dependent concentration.
WB 1/100000. This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.
ICC/IF 1/10000.
IHC (PFA fixed) Use at an assay dependent concentration.

靶标

Anti-MAP2 antibody 图像

  • PFA-fixed, paraffin embedded sections of sheep cerebellum were stained for MAP2 with ab5392 at 1/2000 dilution in immunohistochemical analysis. Goat Anti-Chicken IgY H&L (Biotin) (ab6876) was used as secondary antibody at 1/200 dilution.

     

  • Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/2000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.

  • ab5392 staining MAP2 in murine brain tissue sections by Immunohistochemistry (PFA fixed).
    Boiling in citrate-buffer was used as antigen retrieval method. ab5392 used at a 1/2000 dilution for 12 hours. The secondary used was an Alexa-Fluor 555 conjugated goat anti-chicken polyclonal used at a 1/400 dilution.

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  • All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution

    Lane 1 : Adult rat brain lysate
    Lane 2 : Embryonic E20 rat brain lysate
    Lane 3 : Adult mouse brain lysate
  • ab5392 detecting MAP2 recombinant protein by direct ELISA. Mouse recombinat protien was coated on to microplate in carbonate coating buffer pH 9.6 for 1 hour at 37°C, Plate were blocked with 3% BSA for 1 hour at 37°C and incubated with the primary antibody (1/5000 in PBS + 1% Tween-20) for 1 hour at 37°C. An alkaline phosphatase Goat anti-chicken IgG polyclonal (1/100000) was used as the secondary antibody.

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  • PFA-fixed, paraffin embedded sections of rat brain were stained for MAP2 with ab5392 at 1/4000 dilution in immunohistochemical analysis. Goat Anti-Chicken IgY H&L (Biotin) (ab6876) was used as secondary antibody at 1/200 dilution.

     

  • ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi.
  • Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).
  • ab5392 staining MAP2 in rat spinal cord tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and permeablized with 50% Ethanol for 30 minutes. The sample was incubated with primary antibody (1/250 in PBS + 0.3% Triton X-100) at 4°C for 48 hours. An Alexa Fluor® 488-conjugated goat anti-chicken IgY polyclonal (1/1000) was used as the secondary antibody.

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  • PFA-fixed, paraffin embedded sections of dog brain were stained for MAP2 with ab5392 at 1/2000 dilution in immunohistochemical analysis. Goat Anti-Chicken IgY H&L (Biotin) (ab6876) was used as secondary antibody at 1/200 dilution.

  • ab5392 at 1/5000 staining PFA fixed free floating sections of mouse brain.  An AlexaFluor® 633 conjugated goat anti-chicken antibody was used as the secondary.

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  • ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody.

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  • Anti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg

    Secondary
    HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 70,280 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

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Anti-MAP2 antibody (ab5392)参考文献

This product has been referenced in:
  • Kreiner G  et al. Lack of riluzole efficacy in the progression of the neurodegenerative phenotype in a new conditional mouse model of striatal degeneration. PeerJ 5:e3240 (2017). IHC-P ; Mouse . Read more (PubMed: 28462043) »
  • Yuan J  et al. M2 microglia promotes neurogenesis and oligodendrogenesis from neural stem/progenitor cells via the PPAR? signaling pathway. Oncotarget 8:19855-19865 (2017). ICC/IF ; Mouse . Read more (PubMed: 28423639) »

See all 172 Publications for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Permeabilization
Yes - 50% Ethanol 30 min
Fixative
Paraformaldehyde
Username

Mr. Tom Owen

Verified customer

提交于 Apr 08 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA PH9.0
Permeabilization
Yes - 0.1% TritonX-100
Specification
Brain
Blocking step
Cas-Block(Invitrogen) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 25 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Temporal Cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Specification
Temporal Cortex
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Kathleen

Verified customer

提交于 Feb 22 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Olfactory stem cells)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Olfactory stem cells)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

提交于 Nov 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Olfactory stem cells GFP)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells GFP
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 18 2016

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (NSCs (Neural Stem cells))
Permeabilization
Yes - tritonx100
Specification
NSCs (Neural Stem cells)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Kyungjoo Seong

Verified customer

提交于 Nov 02 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Neuron)
Permeabilization
Yes - TX100 0.05%
Specification
Neuron
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 20 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Ferret Tissue sections (P0-ferret-parietal cortex)
Permeabilization
Yes - 0.5% Triton in 1x PBS for 1 hour5% Triton in 1x PBS for 1 hour
Specification
P0-ferret-parietal cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Francis Djankpa

Verified customer

提交于 Apr 29 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Ferret Tissue sections (parietal cortex in Brain coronal slices)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1x Citrate Buffer
Permeabilization
Yes - 0.5% Triton in 1x PBS for 1 hour
Specification
parietal cortex in Brain coronal slices
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Francis Djankpa

Verified customer

提交于 Mar 03 2016

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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