Anti-MAP1LC3A抗体[EP1983Y] - Autophagosome Marker (ab52768)


  • 产品名称Anti-MAP1LC3A抗体[EP1983Y] - Autophagosome Marker
    参阅全部 MAP1LC3A 一抗
  • 描述
    兔单克隆抗体[EP1983Y] to MAP1LC3A - Autophagosome Marker
  • 特异性This antibody reacts with MAP1LC3A.
  • 经测试应用适用于: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues on the C terminus of human MAP1LC3A.

  • 阳性对照
    • WB: Hela cell lysate IHC-P: Human breast carcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-MAP1LC3A antibody (Alexa Fluor® 488) - Autophagosome Marker [EP1983Y] (ab185036)



Our Abpromise guarantee covers the use of ab52768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
IP 1/40.
Flow Cyt 1/40 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/500.


  • 功能Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • 组织特异性Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
  • 序列相似性Belongs to the MAP1 LC3 family.
  • 翻译后修饰The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • 细胞定位Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATG8E antibody
    • Autophagy-related protein LC3 A antibody
    • Autophagy-related ubiquitin-like modifier LC3 A antibody
    • LC3 antibody
    • LC3A antibody
    • MAP1 light chain 3 like protein 1 antibody
    • MAP1 light chain 3-like protein 1 antibody
    • MAP1A/1B light chain 3 A antibody
    • MAP1A/MAP1B LC3 A antibody
    • MAP1A/MAP1B light chain 3 A antibody
    • MAP1ALC3 antibody
    • MAP1BLC3 antibody
    • Map1lc3a antibody
    • Microtubule associated proteins 1A/1B light chain 3 antibody
    • Microtubule-associated protein 1 light chain 3 alpha antibody
    • Microtubule-associated proteins 1A and 1B, light chain 3 antibody
    • Microtubule-associated proteins 1A/1B light chain 3A antibody
    • MLP3A_HUMAN antibody
    see all

Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker 图像

  • Immunocytochemistry/Immunofluorescence analysis of C6 (rat glioma) labelling MAP1LC3A with purified ab52768 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

  • Overlay histogram showing SHSY-5Y cells stained with ab52768 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52768, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768) at 1/10000 dilution + Hela cell lysate at 10 µg

    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 14 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry/Immunofluorescence analysis of human keratinocytes (pannel A) and VZV-infected melanoma cells (panel E) labelling MAP1LC3A with ab52768.

  • Immunohistochemical staining of MAP1LC3A in paraffin embedded human breast carcinoma using ab52768 at a 1/100 dilution.

Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768)参考文献

This product has been referenced in:
  • Buckingham EM  et al. Nuclear LC3-positive puncta in stressed cells do not represent autophagosomes. Biotechniques 57:241-4 (2014). ICC/IF ; Human . Read more (PubMed: 25399676) »
  • Jackson W  et al. Visualization and quantitation of abundant macroautophagy in virus-infected cells by confocal three-dimensional fluorescence imaging. J Virol Methods 193:244-50 (2013). ICC/IF ; Human . Read more (PubMed: 23792686) »

See all 2 Publications for this product

Product Wall

Thank you for your patience. I have heard back from the lab with the following information: 1) This antibody binds to the uncleaved and cleaved form of LC3, LC3-I. 2) It should recognize two isoforms, LC3-I and LC3-II. One of our scientists r...

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Thank you for your email. Yes, it went directly into my inbox. Thank you for your kind words. To answer your questions: I have checked the database entry for the human protein: http://www.uniprot.org/uniprot/Q9H492 and am not able to find informa...

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