概述

  • 产品名称Anti-MAP1LC3A抗体
    参阅全部 MAP1LC3A 一抗
  • 描述
    兔多克隆抗体to MAP1LC3A
  • 特异性Recognizes human and rat LC3A-I and LC3A-II by immunoblotting and immunoprecipitation (~18 kDa and ~16 kDa, respectively), and mouse LC3A-I (~18 kDa). The detection of mouse LC3A-II may require specific cell treatment and/or long exposure.
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    PSDRPFKQRRSFADR

    conjugated to KLH by a Cysteine residue linker, corresponding to amino acids 2-15 of Human MAP1LC3A

  • 阳性对照
    • Whole brain. U87 cells.

性能

应用

Our Abpromise guarantee covers the use of ab62720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 16-18 kDa (predicted molecular weight: 16-18 kDa).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • 组织特异性Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
  • 序列相似性Belongs to the MAP1 LC3 family.
  • 翻译后修饰The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • 细胞定位Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATG8E antibody
    • Autophagy-related protein LC3 A antibody
    • Autophagy-related ubiquitin-like modifier LC3 A antibody
    • LC3 antibody
    • LC3A antibody
    • MAP1 light chain 3 like protein 1 antibody
    • MAP1 light chain 3-like protein 1 antibody
    • MAP1A/1B light chain 3 A antibody
    • MAP1A/MAP1B LC3 A antibody
    • MAP1A/MAP1B light chain 3 A antibody
    • MAP1ALC3 antibody
    • MAP1BLC3 antibody
    • Map1lc3a antibody
    • Microtubule associated proteins 1A/1B light chain 3 antibody
    • Microtubule-associated protein 1 light chain 3 alpha antibody
    • Microtubule-associated proteins 1A and 1B, light chain 3 antibody
    • Microtubule-associated proteins 1A/1B light chain 3A antibody
    • MLP3A_HUMAN antibody
    see all

Anti-MAP1LC3A antibody 图像

  • ab62720 staining MAP1LC3A in Human HuVEC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-MAP1LC3A antibody (ab62720)参考文献

This product has been referenced in:
  • Koukourakis MI  et al. Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines. PLoS One 10:e0137675 (2015). WB ; Human . Read more (PubMed: 26378792) »
  • Kalamida D  et al. Important role of autophagy in endothelial cell response to ionizing radiation. PLoS One 9:e102408 (2014). WB, ICC/IF ; Human . Read more (PubMed: 25010689) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma cell lines)
Gel Running Conditions Non-reduced Denaturing (12.5)
Loading amount 40 µg
Specification Glioblastoma cell lines
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Mr. Achilleas Mitrakas

Verified customer

提交于 May 06 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 17 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (HuVEC)
Specification HuVEC
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Nov 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"