Mouse, Rat, Cow, Pig, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse Map17.
(Peptide available as ab37556.)
- This antibody gave a positive signal in the Recombinant Human Map17 protein.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 33 kDa corresponding to the molecular weight of recombinant Map17 (predicted molecular weight: 12 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
MAP17 is a 17 kDa membrane-associated protein expressed abundantly in carcinomas arising from kidney, colon, lung, and breast. In normal tissues it is expressed in significant amounts in the kidney.
- 17 kDa membrane associated protein antibody
- DD96 antibody
- Epithelial protein up regulated in carcinoma membrane associated protein 17 antibody
Western blot - Anti-Map17 antibody (ab31405)
Anti-Map17 antibody (ab31405) at 1 µg/ml + Map17 Human Recombinant Protein at 0.1 µgSecondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.Predicted band size:
12 kDaObserved band size:
33 kDa (why is the actual band size different from the predicted?
ab31405 gave a positive signal against the partial recombinant Map17 protein with GST tag which has an expected molecular weight of 33 kDa.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"