ab27157 reacts specifically with MKS1 from plants as well as recombinant MKS1 expressed in E-Coli. Depending upon extract, other non-specific bands may occasionally be seen. The antibody recognizes MKS1 regardless of the phosphorylation status of MKS1.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Predicted molecular weight: 27 kDa.
MAP kinase substrate 1 (MKS1) is phosphorylated by MAP kinase 4 in Arabidopsis. MAP kinase 4 is involved in regulating plant defences against pathogens. It interacts with WRKY transcriptional regulators.