Anti-Mannose Receptor抗体[15-2] (ab8918)

概述

  • 产品名称Anti-Mannose Receptor抗体[15-2]
    参阅全部 Mannose Receptor 一抗
  • 描述
    小鼠单克隆抗体[15-2] to Mannose Receptor
  • 经测试应用适用于: Flow Cyt, IHC-Fr, WB, IHC-R, IHC-FoFr, Inhibition Assay, ICC/IFmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Horse, Human
  • 免疫原

    Purified human mannose receptor from human placental tissue.

  • 表位Stains extracellular domain of Mannose Receptor.
  • 阳性对照
    • Macrophages IHC: liver endothelial cells and Kupffer cells, spermatids, spermatozoa
  • 常规说明


    The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明0.2 µm filtered antibody solution
  • Primary antibody说明The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.
  • 克隆单克隆
  • 克隆编号15-2
  • 骨髓瘤unknown
  • 同种型IgG1
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/50.
WB 1/50. Detects a band of approximately 175 kDa (predicted molecular weight: 166 kDa). This antibody can be use for ligand-receptor intervention studies.
IHC-R Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration. Antibody 15-2 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor.
ICC/IF Use at an assay dependent concentration.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
    • 序列相似性Contains 8 C-type lectin domains.
      Contains 1 fibronectin type-II domain.
      Contains 1 ricin B-type lectin domain.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • bA541I19.1 antibody
      • C type lectin domain family 13 member D antibody
      • C-type lectin domain family 13 member D antibody
      • CD 206 antibody
      • CD206 antibody
      • CD206 antigen antibody
      • CLEC13D antibody
      • CLEC13DL antibody
      • Macrophage mannose receptor 1 antibody
      • Macrophage mannose receptor 1 like protein 1 antibody
      • Macrophage mannose receptor antibody
      • Mannose receptor C type 1 antibody
      • Mannose receptor C type 1 like 1 antibody
      • MMR antibody
      • MRC 1 antibody
      • MRC1 antibody
      • MRC1_HUMAN antibody
      • MRC1L1 antibody
      • OTTHUMP00000045206 antibody
      see all

    Anti-Mannose Receptor antibody [15-2] 图像

    • ab8918 staining Mannose Receptor in human mammary gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 70% Ethanol and blocking for 10 minutes at RT was performed. The sample was incubated with primary antibody (1/10) for 1 hour. A HRP-conjugated mouse polyclonal to mouse IgG was used undiluted as secondary antibody.

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    • ab8918 staining the Mannose Receptor in Human nasal polyp and tonsil tissue sections by Immunohistochemistry (resin-embedded sections). The tissue was fixed in acetone and inhibitors and embedded into GMA resin. Samples were incubated with primary antibody (1/400 in Tris buffered saline) for 18 hours at 21°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/1000) was used as the secondary antibody.

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    • Immunohistochemical analysis of murine uterus tissue sections, staining Mannose Receptor with ab8918.

      Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/40 in 1% BSA) for 1.5 hours. An undiluted HRP-conjugated rabbit anti-mouse polyclonal IgG was used as the secondary antibody.

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    • ab8918 staining Mannose Receptor in human endometriosis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/10 in PBS + 1% NGS) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    Anti-Mannose Receptor antibody [15-2] (ab8918)参考文献

    This product has been referenced in:
    • Mou W  et al. Expression of Sox2 in breast cancer cells promotes the recruitment of M2 macrophages to tumor microenvironment. Cancer Lett 358:115-23 (2015). Read more (PubMed: 25444903) »
    • Wu Z  et al. The prevention of colorectal anastomotic leakage with tissue adhesives in a contaminated environment is associated with the presence of anti-inflammatory macrophages. Int J Colorectal Dis 29:1507-16 (2014). Read more (PubMed: 25255850) »

    See all 13 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Sample Human Tissue sections (inflamed human colon)
    Specification inflamed human colon
    Permeabilization No
    Fixative Acetone
    Username

    Abcam user community

    Verified customer

    提交于 Dec 01 2014

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample Human Tissue sections (Endometriosis)
    Specification Endometriosis
    Permeabilization No
    Fixative Acetone
    Username

    Mrs. Aimée-Lys Rusesabagina

    Verified customer

    提交于 Sep 12 2014

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Sample Mouse Tissue sections (Breast tumor)
    Specification Breast tumor
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Dr. Gajendra Katara

    Verified customer

    提交于 Sep 05 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Sample Mouse Cell (Peritoneal macrophages)
    Specification Peritoneal macrophages
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Aug 20 2014

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3 · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample Mouse Tissue sections (Uterus)
    Specification Uterus
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Dr. Mukesh Jaiswal

    Verified customer

    提交于 Nov 12 2013

    Thank you for contacting us.

    Both of these product are tested and guaranteed to work in Flow Cytometry. These are specific against extracellular domain so the permeabilization step is optional; the antibodies would be able to stain the cells w...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Uterus)
    Specification Uterus
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Dr. Mukesh Jaiswal

    Verified customer

    提交于 Sep 21 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (placenta)
    Specification placenta
    Fixative Formaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Varkha Agrawal

    Verified customer

    提交于 Sep 18 2012

    Thank you very much for your interest in our antibodies.

    To our knowledge, ab8918 and ab64693have not been tested in ELISA and also not in sandwichELISA.

    The epitope of ab8918 was not mapped and the same is true for ab117644.
    ...

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    Thank you for your enquiry.

    I am sorry to confirm that as far as we are aware, ab8918 has never been tested in rat or IHC-P. All tested species and applications covered by the guaranteeare specified on our datasheets, and these are updated a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"