Anti-Mannan Binding Lectin抗体[3B6] (ab23457)

概述

  • 产品名称
    Anti-Mannan Binding Lectin抗体[3B6]
    参阅全部 Mannan Binding Lectin 一抗
  • 描述
    小鼠单克隆抗体[3B6] to Mannan Binding Lectin
  • 特异性
    ab23457 is specific for MBL from human serum or plasma. In Western blotting, ab121075 reacts with Human MBL in both its oligomerized state.
  • 经测试应用
    适用于: WB, IHC-Fr, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Mannan Binding Lectin. Human MBL purified from Human donor plasma.

  • 表位
    The epitope is on the head-neck region of the MBL protein chain.
  • 阳性对照
    • Human liver, Human cancerous liver and Human brain tissues.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    3B6
  • 骨髓瘤
    x63-Ag8.653
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 26 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/250.
ELISA 1/16000.

靶标

  • 功能
    Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
  • 组织特异性
    Plasma protein produced mainly in the liver.
  • 疾病相关
    Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections.
  • 序列相似性
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COLEC 1 antibody
    • COLEC1 antibody
    • Collectin-1 antibody
    • HSMBPC antibody
    • Lectin, mannose-binding, soluble, 2 antibody
    • Mannan binding lectin antibody
    • Mannan binding protein antibody
    • Mannan-binding protein antibody
    • Mannose binding lectin (protein C) 2 soluble antibody
    • Mannose binding lectin (protein C) 2, soluble (opsonic defect) antibody
    • Mannose binding lectin (protein C) 2, soluble antibody
    • Mannose binding lectin 2 soluble antibody
    • Mannose binding lectin 2, soluble (opsonic defect) antibody
    • Mannose binding lectin antibody
    • Mannose binding lectin protein C2 soluble opsonic defect antibody
    • Mannose binding protein antibody
    • Mannose binding protein C antibody
    • Mannose binding protein C precursor antibody
    • Mannose binding protein, serum antibody
    • Mannose-binding lectin antibody
    • Mannose-binding protein C antibody
    • MBL 2 antibody
    • MBL antibody
    • MBL2 antibody
    • MBL2_HUMAN antibody
    • MBL2D antibody
    • MBP 1 antibody
    • MBP antibody
    • MBP C antibody
    • MBP-C antibody
    • MBP1 antibody
    • MBPB antibody
    • MBPC antibody
    • MBPD antibody
    • MGC116832 antibody
    • MGC116833 antibody
    • Opsonic defect antibody
    • protein C antibody
    • Soluble mannose binding lectin antibody
    see all

Anti-Mannan Binding Lectin antibody [3B6] 图像

  • ab23457 at 1 mg/ml staining human liver by IHC-P
  • ab23457 staining MBL in Paraffin-embedded Human Liver tissue by Immunohistochemistry.

  • ab23457 staining MBL in Paraffin-embedded Human cancerous liver tissue by Immunohistochemistry.

Anti-Mannan Binding Lectin antibody [3B6] (ab23457)参考文献

This product has been referenced in:
  • Yadav AK  et al. Differential expression of collectins in human placenta and role in inflammation during spontaneous labor. PLoS One 9:e108815 (2014). WB ; Human . Read more (PubMed: 25303045) »
  • Debiec H  et al. Recurrent membranous nephropathy in an allograft caused by IgG3? targeting the PLA2 receptor. J Am Soc Nephrol 23:1949-54 (2012). Read more (PubMed: 23123401) »

See all 13 Publications for this product

Product Wall

Thank you for this additional information. A few suggestions just to follow a protocol a little more similar to how we have tested. We generally recommend blocking in 5% milk in TBST and incubating the primary antibody overnight at 4 degrees. You may a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain tissue)
Specification
brain tissue
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Vlady Pavlova

Verified customer

提交于 Aug 15 2006

Thank you for your enquiry. The antibody has been tested in IHC in human liver and brain sections, but we do not have those images unfortunately. The reference Holmskov UL Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59 (20...

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Thank you for your enquiry. I have been informed by our lab that the antiserum was prepared in 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide. Please do not hesitate to contact me should you require further assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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