Anti-Mannan Binding Lectin抗体[14D12] (ab106046)

概述

  • 产品名称Anti-Mannan Binding Lectin抗体[14D12]
    参阅全部 Mannan Binding Lectin 一抗
  • 描述
    大鼠单克隆抗体[14D12] to Mannan Binding Lectin
  • 特异性It is recommended that solutions with a calcium concentration of 1 mM are used as ab106046 is a calcium-dependent antibody.
  • 经测试应用适用于: WB, ELISA, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Purified Mouse Mannan Binding Lectin-C emulsified in complete Freund's adjuvant.

  • 阳性对照
    • Mouse Serum and kidney tissue

性能

应用

Our Abpromise guarantee covers the use of ab106046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10. Predicted molecular weight: 26 kDa. On a 12% non-reducing SDS-PAGE a prominent band of ~50Kda is detected. Under reducing conditions a 26 KDa band is detected.
ELISA Use at an assay dependent dilution. Biotinylated ab106046 was used as detector in ELISA at 1µg/ml TBS/Tween-20/Ca.
IHC-Fr 1/10. Cryostat sections (7µm) of mouse tissue were acetone fixed and stained o/n at 4C in TBS/2% goat serum
ICC/IF Use at an assay dependent dilution. Hyphae of C. albicans were fixed on poly-l-lysine coated slides with ice-cold acetone. Slides were incubated for 1h at RT with ab106046.

靶标

  • 功能Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
  • 组织特异性Plasma protein produced mainly in the liver.
  • 疾病相关Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections.
  • 序列相似性Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COLEC 1 antibody
    • COLEC1 antibody
    • Collectin-1 antibody
    • HSMBPC antibody
    • Lectin, mannose-binding, soluble, 2 antibody
    • Mannan binding lectin antibody
    • Mannan binding protein antibody
    • Mannan-binding protein antibody
    • Mannose binding lectin (protein C) 2 soluble antibody
    • Mannose binding lectin (protein C) 2, soluble (opsonic defect) antibody
    • Mannose binding lectin (protein C) 2, soluble antibody
    • Mannose binding lectin 2 soluble antibody
    • Mannose binding lectin 2, soluble (opsonic defect) antibody
    • Mannose binding lectin antibody
    • Mannose binding lectin protein C2 soluble opsonic defect antibody
    • Mannose binding protein antibody
    • Mannose binding protein C antibody
    • Mannose binding protein C precursor antibody
    • Mannose binding protein, serum antibody
    • Mannose-binding lectin antibody
    • Mannose-binding protein C antibody
    • MBL 2 antibody
    • MBL antibody
    • MBL2 antibody
    • MBL2_HUMAN antibody
    • MBL2D antibody
    • MBP 1 antibody
    • MBP antibody
    • MBP C antibody
    • MBP-C antibody
    • MBP1 antibody
    • MBPB antibody
    • MBPC antibody
    • MBPD antibody
    • MGC116832 antibody
    • MGC116833 antibody
    • Opsonic defect antibody
    • protein C antibody
    • Soluble mannose binding lectin antibody
    see all

Anti-Mannan Binding Lectin antibody [14D12] 图像

  • ab106046 detecting Mannan Binding Lectin deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. Mannan Binding Lectin was detected in and around invading macrophages invading the intima (insert). Mannan Binding Lectin bound at sites of necrosis (upper right corner). No Mannan Binding Lectin binding was shown in the media or on fibrous caps covering the thickened intima.

Anti-Mannan Binding Lectin antibody [14D12] (ab106046)参考文献

This product has been referenced in:
  • Garland DL  et al. Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23943789) »
  • Matthijsen RA  et al. Macrophage-specific expression of mannose-binding lectin controls atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation 119:2188-95 (2009). Read more (PubMed: 19380618) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"