Recombinant fragment, corresponding to a sequence within amino acids 414-670 of Human MAN1B1.
Hela (whole cell), A431 and HepG2 lysates; SCC25 (a human head and neck squamous cell carcinoma line) tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
1/100 - 1/250.
MAN1B1 (Mannosidase, alpha, class 1B, member 1) catalyzes the first mannose-trimming step in maturation of Asn-linked oligosaccharides. It trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2), specifically converting it to Man8GlcNAc isomer B. MAN1B1 may be involved in glycoprotein quality control since it is important to target misfolded glycoproteins for degradation.
Endoplasmic reticulum membrane; Single-pass type II membrane protein.
Alpha 1 2 mannosidase antibody
Endoplasmic reticulum alpha mannosidase 1 antibody
Endoplasmic reticulum mannosyl oligosaccharide 1 2 alpha mannosidase 1 antibody
Western blot - MAN1B1 antibody (ab95975)
Anti-MAN1B1 antibody (ab95975) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 80 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAN1B1 antibody (ab95975)
ab95975 at 1/100 dilution staining MAN1B1 in SCC25 by Immunohistochemistry, Paraffin-embedded tissue.
has not yet been referenced specifically in any publications.
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