概述

  • 产品名称Anti-Mammaglobin A抗体
    参阅全部 Mammaglobin A 一抗
  • 描述
    兔多克隆抗体to Mammaglobin A
  • 特异性No cross-reactivity to lipophilin A, lipophilin B (BU101), lipophilin C (lacryglobin, mammaglobin B), or uteroglobin (Clara cell protein, Clara cell 10 kDa protein) by Western blot analysis of bacterially produced recombinant proteins.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: EVFMQLIYDSSLCDLF conjugated to KLH, corresponding to C terminal amino acids 78 - 93 of Human mammaglobin.

  • 阳性对照
    • Human breast cancer cells.

性能

应用

Our Abpromise guarantee covers the use of ab18517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa.
IHC-P Use at an assay dependent concentration.

靶标

  • 组织特异性Mammary gland specific. Over-expressed in breast cancer.
  • 序列相似性Belongs to the secretoglobin family. Lipophilin subfamily.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Mammaglobin 1 antibody
    • Mammaglobin-1 antibody
    • Mammaglobin-A antibody
    • MGB1 antibody
    • Scgb2a2 antibody
    • Secretoglobin family 2A member 2 antibody
    • SG2A2 antibody
    • SG2A2_HUMAN antibody
    • UGB2 antibody
    see all

Anti-Mammaglobin A antibody 图像

  • IHC image of ab18517 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18517, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Mammaglobin A antibody (ab18517)参考文献

This product has been referenced in:
  • Long W  et al. SRC-3Delta4 mediates the interaction of EGFR with FAK to promote cell migration. Mol Cell 37:321-32 (2010). IHC-P ; Mouse . Read more (PubMed: 20159552) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"