RabMAb

Anti-Mammaglobin A抗体[31A5] (ab82203)

概述

  • 产品名称
    Anti-Mammaglobin A抗体[31A5]
    参阅全部 Mammaglobin A 一抗
  • 描述
    兔单克隆抗体[31A5] to Mammaglobin A
  • 特异性
    Reacts with human mammaglobin A, no cross-reaction with lypophilin B and lypophilin C (mammaglobin B).
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
  • 免疫原

    Recombinant full length protein (Human) Mammaglobin A protein

  • 阳性对照
    • Breast carcinoma, skin (eccrine sweat glands).

性能

应用

Our Abpromise guarantee covers the use of ab82203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明
    ELISA: Use at an assay dependent dilution.

    IHC-P: Use at a concentration of 1 µg/ml. Perform antigen retrieval step using Citrate buffer, or buffered EDTA, or Glycol based solution before commencing with IHC staining protocol.
    This antibody is suitable for the detection of occult mamma carcinoma cells in sentinel lymph nodes and in peripheral blood. The overall sensitivity of mammaglobin detection on paraffin sections of mamma carcinoma was reported to be >80% (Fanger et al. 2002; Han et al. 2003).

    WB: Use at an assay dependent dilution. See PubMed ID: 14521461

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    图片

    • ab82203 at 1µg/ml staining Mammaglobin A in sentinel lymph nodes in metastasis of breast carcinoma.
    • ab82203 at 1µg/ml staining Mammaglobin A over-expression in breast carcinoma in situ.

    文献

    This product has been referenced in:
    • Plante I  et al. Cx43 suppresses mammary tumor metastasis to the lung in a Cx43 mutant mouse model of human disease. Oncogene : (2010). IHC-P ; Mouse . Read more (PubMed: 21151177) »
    • Han JH  et al. Mammaglobin expression in lymph nodes is an important marker of metastatic breast carcinoma. Arch Pathol Lab Med 127:1330-4 (2003). WB, IHC-P, ELISA ; Human, Mouse, Monkey . Read more (PubMed: 14521461) »

    See all 3 Publications for this product

    客户评价及客户问答

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (4T1 breast tumor metastases in lung and liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    Yes - Triton
    Specification
    4T1 breast tumor metastases in lung and liver
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 May 27 2015

    Thank you for getting back to me and for confirming the order details. Currently, ab82203 is the only antibody against Mammaglobin A which has been tested for cross-reaction with mouse samples. Although, we have some other antibodies against the same t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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