The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500.
Epitope exposure is recommended. Epitope exposure with Tris-EDTA pH9.0 buffer will enhance staining.
1/50. PubMed: 25229469
功能Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Potentiates activation by NOTCH3 and NOTCH4 more efficiently than MAML1 or MAML3.
组织特异性Widely expressed with high levels detected in placenta, salivary gland and skeletal muscle.
疾病相关Note=A chromosomal aberration involving MAML2 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with CRTC1/MECT1. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
序列相似性Belongs to the mastermind family.
结构域The C-terminal domain is required for transcriptional activation.
细胞定位Nucleus speckle. Nuclear, in a punctate manner.