概述

  • 产品名称Anti-MAML1抗体
    参阅全部 MAML1 一抗
  • 描述
    兔多克隆抗体to MAML1
  • 特异性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 75-125 of Human MAML1

  • 阳性对照
    • Whole cell lysate from HeLa cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab70488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
IP Use at 1-4 µg/mg of lysate.

靶标

  • 功能Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
  • 组织特异性Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
  • 序列相似性Belongs to the mastermind family.
  • 结构域The C-terminal region is required for transcriptional activation.
  • 细胞定位Nucleus speckle. Nuclear, in a punctate manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Mam-1 antibody
    • Mam1 antibody
    • MAML 1 antibody
    • Maml1 antibody
    • MAML1_HUMAN antibody
    • Mastermind like 1 antibody
    • Mastermind-like protein 1 antibody
    see all

Anti-MAML1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MAML1 with ab70488 at 1/1000 (1µg/ml). Detection: DAB.
  • Anti-MAML1 antibody (ab70488) at 1 µg/ml + Whole cell lysate from HeLa cells at 50 µg
    Developed using the ECL technique

    Predicted band size : 108 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of Human MAML1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70488 at 3 µg/mg lysate for IP.
    Chemiluminescence with exposure times of 30 seconds.

Anti-MAML1 antibody (ab70488)参考文献

ab70488 has not yet been referenced specifically in any publications.

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