概述

  • 产品名称Anti-MAML1抗体
    参阅全部 MAML1 一抗
  • 描述
    兔多克隆抗体to MAML1
  • 特异性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human MAML1

  • 阳性对照
    • Whole cell lysate from HeLa cells and 293T cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab70486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 1-4µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
    • 组织特异性Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
    • 序列相似性Belongs to the mastermind family.
    • 结构域The C-terminal region is required for transcriptional activation.
    • 细胞定位Nucleus speckle. Nuclear, in a punctate manner.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Mam-1 antibody
      • Mam1 antibody
      • MAML 1 antibody
      • Maml1 antibody
      • MAML1_HUMAN antibody
      • Mastermind like 1 antibody
      • Mastermind-like protein 1 antibody
      see all

    Anti-MAML1 antibody 图像

    • All lanes : Anti-MAML1 antibody (ab70486) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 108 kDa


      Exposure time : 30 seconds
    • Detection of Human MAML1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70486 at 3 µg/mg lysate for IP.
      Chemiluminescence with exposure times of 30 seconds.

    Anti-MAML1 antibody (ab70486)参考文献

    ab70486 has not yet been referenced specifically in any publications.

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