概述

  • 产品名称
  • 描述
    兔多克隆抗体to MAML1
  • 特异性
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human MAML1

  • 阳性对照
    • Whole cell lysate from HeLa cells and 293T cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 1-4µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
    • 组织特异性
      Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
    • 序列相似性
      Belongs to the mastermind family.
    • 结构域
      The C-terminal region is required for transcriptional activation.
    • 细胞定位
      Nucleus speckle. Nuclear, in a punctate manner.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Mam-1 antibody
      • Mam1 antibody
      • MAML 1 antibody
      • Maml1 antibody
      • MAML1_HUMAN antibody
      • Mastermind like 1 antibody
      • Mastermind-like protein 1 antibody
      see all

    图片

    • All lanes : Anti-MAML1 antibody (ab70486) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 108 kDa


      Exposure time : 30 seconds
    • Detection of Human MAML1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70486 at 3 µg/mg lysate for IP.
      Chemiluminescence with exposure times of 30 seconds.

    文献

    ab70486 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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