概述
- 产品名称Anti-MAML1抗体
参阅全部 MAML1 一抗 - 描述兔多克隆抗体to MAML1
- 经测试应用适用于: IP, WB, ICC/IFmore details
- 种属反应性与反应: Rat, Human
预测可用于: Mouse, Horse
- 免疫原
Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus MAML1.
(Peptide available as ab66491.)
- 阳性对照
- This antibody gave a positive signal in the following Lysates; SW480, HepG2, HeLa and Rat Thymus Tissue Lysate.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab65090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 117 kDa (predicted molecular weight: 108 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
- 功能Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
- 组织特异性Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
- 序列相似性Belongs to the mastermind family.
- 结构域The C-terminal region is required for transcriptional activation.
- 细胞定位Nucleus speckle. Nuclear, in a punctate manner.
- Information by UniProt
-
数据库链接
- Entrez Gene: 9794 Human
- Entrez Gene: 103806 Mouse
- Entrez Gene: 303101 Rat
- Omim: 605424 Human
- SwissProt: Q92585 Human
- SwissProt: Q6T264 Mouse
- Unigene: 631951 Human
- Unigene: 51116 Mouse
-
别名
- Mam-1 antibody
- Mam1 antibody
- MAML 1 antibody
see all
Anti-MAML1 antibody 图像
-
Immunoprecipitation - Anti-MAML1 antibody (ab65090)MAML1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MAML1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65090.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 117kDa; MAML1
-
Western blot - MAML1 antibody (ab65090)All lanes : Anti-MAML1 antibody (ab65090) at 1 µg/ml
Lane 1 :SW480 whole cell lysate (ab3957)
Lane 2 : Rat Thymus Tissue Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 108 kDa
Observed band size : 117 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - MAML1 antibody (ab65090)ICC/IF image of ab65090 stained HeLa cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65090 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml.
Anti-MAML1 antibody (ab65090)参考文献
ab65090 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"