Anti-MAMDC2抗体(ab121710)
Key features and details
- Rabbit polyclonal to MAMDC2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MAMDC2抗体
参阅全部 MAMDC2 一抗 -
描述
兔多克隆抗体to MAMDC2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to amino acids 322-419 (FSPVHCQNQT ELLFSAVEAS CNFEQDLCNF YQDKEGPGWT RVKVKPNMYR AGDHTTGLGY YLLANTKFTS QPGYIGRLYG PSLPGNLQYC LRFHYAIY) of Human MAMDC2.
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阳性对照
- IHC: Human cervix, skin and gastrointestinal tissues. WB: HEK293T cell lysate ICC/IF: Human cell line U-2 OS shows
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121710于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Contains 4 MAM domains. -
翻译后修饰
O-glycosylated. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 256691 Human
- Omim: 612879 Human
- SwissProt: Q7Z304 Human
- Unigene: 547172 Human
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别名
- MAM domain-containing protein 2 antibody
- MAM domain-containing proteoglycan antibody
- MAMC2_HUMAN antibody
see all
图片
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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & plasma membrane.
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Lanes 2-3 : Anti-MAMDC2 antibody (ab121710)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing MAMDC2 with a C-terminal myc-DDK tag (~3.1kDa) -
Immunohistochemical analysis of human cervix tissue labeling MAMDC2 with ab121710 at a 1/50 dilution.
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Immunohistochemical analysis of human gastrointestinal tissue labeling MAMDC2 with ab121710 at a 1/50 dilution.
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Immunohistochemical analysis of human skin tissue labeling MAMDC2 with ab121710 at a 1/50 dilution.
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Immunohistochemical analysis of human pancreas tissue labeling MAMDC2 with ab121710 at a 1/50 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121710 尚未被引用在任何文献中。