Anti-Maltose Binding Protein抗体[MBP-17] (HRP) (ab49923)

概述

  • 产品名称Anti-Maltose Binding Protein抗体[MBP-17] (HRP)
    参阅全部 Maltose Binding Protein 一抗
  • 描述
    小鼠单克隆抗体[MBP-17] to Maltose Binding Protein (HRP)
  • 偶联物HRP
  • 特异性This antibody recognizes native as well as denatured reduced forms of purified Maltose Binding Protein (MBP) or MBP fusion proteins.
  • 经测试应用适用于: WB, ELISA, Dot blotmore details
  • 免疫原

    Recombinant full length Maltose Binding Protein fusion protein

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明The immunoglobulin fraction is purified from ascites fluid of the MBP-17 hybridoma and then conjugated with horseradish peroxidase (HRP).
  • 克隆单克隆
  • 克隆编号MBP-17
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 43 kDa.
ELISA Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

靶标

  • 相关性Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • 细胞定位Periplasm
  • 别名
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

Anti-Maltose Binding Protein antibody [MBP-17] (HRP) 图像

  • ab49923 staining Maltose binding protein by ELISA. The sample was blocked in 1% milk for 1 hour at 18°C. The primary was diluted at 1/2000 in PBS-Tween20 buffer and incubated with sample for 1 hour at 18°C.

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Anti-Maltose Binding Protein antibody [MBP-17] (HRP) (ab49923)参考文献

This product has been referenced in:
  • Ishida K  et al. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator. PLoS Negl Trop Dis 8:e3051 (2014). Read more (PubMed: 25078989) »
  • Beck J  et al. Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol 15:430-9 (2013). Read more (PubMed: 23455478) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Saccharomyces cerevisiae Recombinant protein (Recombinant Yeast SUMO protein)
Specification Recombinant Yeast SUMO protein
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Type Direct
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Abcam user community

Verified customer

提交于 May 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (BL21(DE3))
Loading amount 1 µg
Specification BL21(DE3)
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Jul 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Rat Recombinant protein (neuronal)
Specification neuronal
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type Indirect
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Abcam user community

Verified customer

提交于 May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (E.Coli)
Loading amount 0.05 µg
Specification E.Coli
Gel Running Conditions Reduced Denaturing (gradient 4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Jan 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"