Anti-Maltose Binding Protein抗体[MBP-17] (HRP) (ab49923)

概述

  • 产品名称
    Anti-Maltose Binding Protein抗体[MBP-17] (HRP)
    参阅全部 Maltose Binding Protein 一抗
  • 描述
    小鼠单克隆抗体[MBP-17] to Maltose Binding Protein (HRP)
  • 偶联物
    HRP
  • 特异性
    This antibody recognizes native as well as denatured reduced forms of purified Maltose Binding Protein (MBP) or MBP fusion proteins.
  • 经测试应用
    适用于: WB, ELISA, Dot blotmore details
  • 免疫原

    Recombinant full length Maltose Binding Protein fusion protein

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    The immunoglobulin fraction is purified from ascites fluid of the MBP-17 hybridoma and then conjugated with horseradish peroxidase (HRP).
  • 克隆
    单克隆
  • 克隆编号
    MBP-17
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 43 kDa.
ELISA Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

靶标

  • 相关性
    Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • 细胞定位
    Periplasm
  • 别名
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

图片

  • ab49923 staining Maltose binding protein by ELISA. The sample was blocked in 1% milk for 1 hour at 18°C. The primary was diluted at 1/2000 in PBS-Tween20 buffer and incubated with sample for 1 hour at 18°C.

    See Abreview

实验方案

文献

This product has been referenced in:
  • Ishida K  et al. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator. PLoS Negl Trop Dis 8:e3051 (2014). Read more (PubMed: 25078989) »
  • Christian F  et al. PKA phosphorylation of p62/SQSTM1 regulates PB1 domain interaction partner binding. Biochim Biophys Acta 1843:2765-74 (2014). Read more (PubMed: 25110345) »

See all 5 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Saccharomyces cerevisiae Recombinant protein (Recombinant Yeast SUMO protein)
Specification
Recombinant Yeast SUMO protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Type
Direct
Username

Abcam user community

Verified customer

提交于 May 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Loading amount
1 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jul 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rat Recombinant protein (neuronal)
Specification
neuronal
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type
Indirect
Username

Abcam user community

Verified customer

提交于 May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (E.Coli)
Loading amount
0.05 µg
Specification
E.Coli
Gel Running Conditions
Reduced Denaturing (gradient 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jan 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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