Anti-Maltose Binding Protein抗体(ab21144)

概述

  • 产品名称Anti-Maltose Binding Protein抗体
    参阅全部 Maltose Binding Protein 一抗
  • 描述
    兔多克隆抗体to Maltose Binding Protein
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    Maltose binding protein tag
  • 免疫原

    Recombinant full length protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 44 kDa. Dilution optimised using Chromogenic detection.
ELISA 1/10000.

靶标

  • 相关性Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • 细胞定位Periplasm
  • 别名
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

Anti-Maltose Binding Protein antibody 图像

  • Anti-Maltose Binding Protein antibody (ab21144) at 1/5000 dilution

    Secondary
    Anti-Rabbit antibody (Sigma A0545) at 1/16000 dilution
    Developed using the ECL technique

    Observed band size : 47.5 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-Maltose Binding Protein antibody (ab21144)参考文献

This product has been referenced in:
  • Braun J  et al. Rapid identification of regulatory microRNAs by miTRAP (miRNA trapping by RNA in vitro affinity purification). Nucleic Acids Res 42:e66 (2014). Read more (PubMed: 24510096) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cell)
Specification Hela cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.03% Triton X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

提交于 Jan 14 2013

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Application ELISA
Sample Saccharomyces cerevisiae Recombinant protein (Recombinant yeast SUMO protein)
Specification Recombinant yeast SUMO protein
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Type Direct
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Verified customer

提交于 May 12 2011

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Application Western blot
Sample Escherichia coli Recombinant protein (BL21(DE3))
Loading amount 1 µg
Specification BL21(DE3)
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

提交于 Nov 03 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"