Maltose and Glucose Assay试剂盒(ab65335)

概述

  • 产品名称Maltose and Glucose Assay试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型Quantitative
  • 灵敏度
    > 0.01 nmol/well
  • 范围
    0.01 nmol/well - 10 nmol/well
  • 检测时间
    1h 00m
  • 产品概述

    Maltose and Glucose Assay Kit (ab65335) can measure glucose or maltose levels in a variety of biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). The assay is based on the ability of glucose oxidase to specifically oxidize free glucose, producing a product that interacts with the glucose probe. The amount of product can be either colorimetric (spectrophotometry at OD= 570 nm) or fluorometric (Ex/Em = 535/587 nm) method.

    This kit can detect 10 pmol to 10 nmol glucose per assay. It can also be used for isomaltose detection.


    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Glucose (C6H12O6; FW: 180.16) and Maltose (C12H22O11; FW: 342.3) are the main fuel sources to generate the universal energy molecule ATP. Maltose is the major disaccharide that generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a α-1,4-glycosidic linkage, which can be easily converted to two glucoses by α-D-glucosidase.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Maltose (C12H22O11; fW:342.30) is a disaccharide formed from two units of glucose (C6H12O6; FW: 180.16) joined via a a-1,4-glycosidic linkage. Maltose is the major disaccharide generated from hydrolysis of starch and in living organisms, maltose is hydrolyzed by maltase. Once inside the cells, glucose will be processed and ener the glycolysis pathway to ultimately produce enery in form of ATP.

应用

Our Abpromise guarantee covers the use of ab65335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Maltose and Glucose Assay Kit 图像

  • Maltose Standard Curve. Assays were performed using various amounts of Maltose standard according to kit instructions. Circle is maltose with a-D-Glucosidase. Square is maltose without a-D-Glucosidase. Triangle is glucose with 2 times nmol of Maltose.

实验方案

Maltose and Glucose Assay Kit (ab65335)参考文献

ab65335 has not yet been referenced specifically in any publications.

Product Wall

Kit ab65335 has not yet been tested specifically with dog samples. We have not previously used these kits to measure maltase activity, however this may be possible with some protocol optimization.

We do also carry the following two kits which ...

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The alpha-D-Glucosidase included in kit ab65335 is from Saccharomyces cerevisiae.

The following products are meant for detection of total amount of sample, NOT enzyme activity. However, the kits may be able to be used in reverse - meaning that the enzyme in your sample will be used to convert a known amount of substrate (standard) a...

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Product Maltose and Glucose Assay Kit (ab65335) may be suitable for you.   Maltose contains two glucose units joined by an alpha-1,4-glycosidic linkage, which can be easily converted to two glucoses by alpha-D-glucosidase. Glucose oxidase specifically ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"