Maleimide Quantification Assay试剂盒(Fluorometric) (ab112141)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
概述
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产品名称
Maleimide Quantification Assay试剂盒(Fluorometric)
参阅全部 Maleimide 试剂盒 -
检测方法
Fluorescent -
检测类型
Quantitative -
产品概述
ab112141 uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide. The kit provides a sensitive, one-step fluorometric method to detect as little as 10 picomoles of maleimide in a 100 µL assay volume (100 nM; Figure 1). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm. Compared to ab112140, this fluorometric assay is more sensitive, and has less interference from biological samples.
Visit our FAQs page for tips and troubleshooting.
Please note this kit is optimized for the detection of small molecule-based maleimide.
If your sample is maleimide conjugated in protein, then this kit may not be suitable.
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说明
ab112141 should be stored desiccated.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 200 tests Assay Buffer 1 x 25ml DMSO 1 x 200µl MalemGreen indicator 1 vial N-ethylmaleimide Standard 1 x 50µl Reaction Buffer 1 x 500µl -
研究领域
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab112141 被引用在 2 文献中.
- Hurst CH et al. Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods. Biotechniques 62:69-75 (2017). PubMed: 28193150
- Valetti S et al. Peptide conjugation: before or after nanoparticle formation? Bioconjug Chem 25:1971-83 (2014). PubMed: 25313527