Maleimide Quantification Assay试剂盒(Colorimetric) (ab112140)

概述

• 产品名称Maleimide Quantification Assay试剂盒(Colorimetric)
  参阅全部 Maleimide 试剂盒
• 样品类型
  Cell culture extracts, Adherent cells, Suspension cells
• 检测类型Quantitative
• 灵敏度
  10 pg/well
• 产品概述

  ab112140 assay kit quantifies maleimide groups by first reacting a sample with a known amount of thiol present in excess and then assaying the remaining unreacted thiol using 4,4'-DTDP with a molar extinction coefficient of 19,800 M ^-1cm^-1. The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups. This spectrophotometric assay for the determination of maleimide groups is a reverse GSH assay. It takes advantage of the high reactivity of thiols of GSH with the maleimide moiety. Maleimide of the sample is allowed to form a stable thiosuccinimidyl linkage with GSH. After the reaction of the sample is complete, the excess GSH, i.e., the remaining thiols of GSH in the reaction mixture, is estimated by using 4,4'-DTDP. The amount of GSH reacted with the sample is titrated to determine the extent of maleimide. For more sensitive maleimide quantitation, we recommend that you use our fluorimetric kit ab112141 that has higher sensitivity.
  
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• 经测试应用适用于: Functional Studiesmore details

性能

• 存放说明Store at -20°C. Please refer to protocols.

• 组件	标识符	100 tests
  4,4’-DTDP	Component B	1 vial
  Assay Buffer		1 x 50ml
  DMSO		1 x 1ml
  MEA	Component A	1 vial

• 研究领域
  • Cell Biology
  • Other Antibodies
  • Drugs and Chemicals

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