概述

  • 产品名称Anti-MAK抗体
    参阅全部 MAK 一抗
  • 描述
    兔多克隆抗体to MAK
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 575 and 624 (WNTKTGRGQFSGRTYNPTAKNLNIVNRAQPIPSVHGRTDWVAKYGGHR) of Human MAK (NP_005897).

  • 阳性对照
    • Hela cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

ICC/IF 1/100.

靶标

  • 功能Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
  • 组织特异性Expressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines. Isoform 1 is expressed in kidney, testis, lung, trachea, and retina. Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors.
  • 疾病相关Defects in MAK are the cause of retinitis pigmentosa type 62 (RP62) [MIM:614181]. RP62 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated. Phosphorylated on serine and threonine residues.
  • 细胞定位Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Midbody. Cell projection > cilium > photoreceptor outer segment. Photoreceptor inner segment. Localized in both the connecting cilia and the outer segment axonemes (By similarity). Localized uniformly in nuclei during interphase, to the mitotic spindle and centrosomes during metaphase and anaphase, and also to midbody at anaphase until telophase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • intestinal cell kinase antibody
    • dJ417M14.2 antibody
    • fj04c02 antibody
    • Ick antibody
    • Mak antibody
    • MAK_HUMAN antibody
    • Male germ cell associated kinase antibody
    • Male germ cell-associated kinase antibody
    • male germ cell-associated kinase retinal-enriched isoform antibody
    • OTTHUMP00000016025 antibody
    • Rck antibody
    • RP62 antibody
    • Serine/threonine protein kinase MAK antibody
    • Serine/threonine-protein kinase MAK antibody
    • testicular secretory protein Li 28 antibody
    • wu:fj04c02 antibody
    • zgc:56603 antibody
    see all

Anti-MAK antibody 图像

  • Anti-MAK antibody (ab80536) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 71 kDa
    Observed band size : 71 kDa
    Gel concentration: 12%
  • All lanes : Anti-MAK antibody (ab80536) at 1 µg/ml

    Lane 1 : Human pancreatic cancer cell line MiaPaca-2
    Lane 2 : Human pancreatic cancer cell line Panc-1


    Predicted band size : 71 kDa
    WB Suggested Anti-CDC45L Antibody (ab82805) Titration: 0.5 µg/ml Positive Control: mouse stem cells

  • Immunofluorescence analysis of Human lung adenocarcinoma cell line A549 staining MAK with ab80536 at 2.5 µg/ml.

Anti-MAK antibody (ab80536)参考文献

ab80536 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab80536.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"