Magnesium Detection试剂盒(ab102506)

概述

  • 产品名称Magnesium Detection试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • 检测类型Quantitative
  • 灵敏度
    40 µM
  • 范围
    2 µM - 15 µM
  • 检测时间
    0h 30m
  • 产品概述

    Magnesium Assay Kit (ab102506) is a simple sensitive assay for quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+. An enzyme linked reaction leads to formation of an intensely colored (?max = 450nm) product whose formation is proportional to Mg2+ concentration. The linear range of the assay is 2-15 nmoles with detection sensitivity ~40 µM.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Magnesium is the 11th most abundant element by mass in the human body. Mg2+ is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg2+ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg2+ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Magnesium is the 11th most abundant element by mass in the human body. Magnesium is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Magnesium is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Magnesium compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.
  • 别名
    • Mg++
    • Mg+2
    • Mg2+

应用

Our Abpromise guarantee covers the use of ab102506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Magnesium Detection Kit 图像

  • Magnesium measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

  • Magnesium measured in biological fluids showing quantity (nmol) per microliter of tested sample

  • Colourimetric standard curve : mean of duplicates (+/-SD) with background reads substracted

  • Magnesium standard curve: Assay is performed according to kit protocol. Vertical dotted lines indicate the lower and upper limits of normal serum Magnesium concentrations.

实验方案

Magnesium Detection Kit (ab102506)参考文献

ab102506 has not yet been referenced specifically in any publications.

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We have rechecked the QC and retested the kit which is working as expected, therefore it is unclear why the kit is not working well in your hands. As this kit is covered by the Abpromise I am happy to offer a r...

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The lab has some additional questions: How was theenzyme reconstituted/stored? Can you also describe how standards were prepared?


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Based on the data you show it looks like the issue is thatno reaction mix or old/expired mix was used as there is not even the hint of a difference between 0 and t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"