Anti-MAGI3抗体[SLP-32] (ab11509)

概述

  • 产品名称Anti-MAGI3抗体[SLP-32]
    参阅全部 MAGI3 一抗
  • 描述
    小鼠单克隆抗体[SLP-32] to MAGI3
  • 特异性MAGI3 (membrane-associated guanylate cyclase of inverted orientation-3) is a member of the family of membrane-associated guanylate kinases (MAGUKs) that comprise peripheral membrane proteins involved in the formation of specialized cell-cell junctions. MAGI3, as well as other proteins in this family are characterized by three types of protein-protein interaction modules: five PDZ domains, an N-terminal guanylate kinase (GK) domain, and a WW domain. The WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, mediates protein-protein interactions through binding to short proline-rich sequences). MAGI3 and PTEN/MMAC together modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions PTEN/MMAC phosphatase to specific subcellular locations that are involved in the regulation of cell proliferation and survival.
  • 经测试应用适用于: ELISA, WB, ICCmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Synthetic peptide, corresponding to C-term amino acids 421-785 of Rat MAGI3.

  • 阳性对照
    • Tested with rat brain extract.

性能

应用

Our Abpromise guarantee covers the use of ab11509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 160 kDa.
ICC Use at an assay dependent dilution.

靶标

  • 功能Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 6 PDZ (DHR) domains.
    Contains 2 WW domains.
  • 翻译后修饰Ubiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex.
  • 细胞定位Cell membrane. Cell junction > tight junction. Nucleus. Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ730K3.2 antibody
    • MAGI-3 antibody
    • magi3 antibody
    • MAGI3_HUMAN antibody
    • Membrane Associated Guanylate kinase Related 3 antibody
    • Membrane-associated guanylate kinase antibody
    • Membrane-associated guanylate kinase inverted 3 antibody
    • RP4-730K3.1 antibody
    • SLIPR antibody
    • WW and PDZ domain-containing protein 3 antibody
    see all

Anti-MAGI3 antibody [SLP-32] (ab11509)参考文献

ab11509 has not yet been referenced specifically in any publications.

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