概述

  • 产品名称Anti-MAGI3抗体
    参阅全部 MAGI3 一抗
  • 描述
    兔多克隆抗体to MAGI3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 716-979 of Human MAGI3 (BC130409).

  • 阳性对照
    • Human fetal brain, fetal kidney and fetal liver lysates; Human fetal testis tissue.

性能

  • 形式Lyophilised:Reconstitute in 200 µl sterile distilled water.
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab118615 is purified by protein affinity column.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 130, 160 kDa (predicted molecular weight: 123, 160 kDa).
IHC-P 1/100 - 1/500.

靶标

  • 功能Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 6 PDZ (DHR) domains.
    Contains 2 WW domains.
  • 翻译后修饰Ubiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex.
  • 细胞定位Cell membrane. Cell junction > tight junction. Nucleus. Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ730K3.2 antibody
    • MAGI-3 antibody
    • magi3 antibody
    • MAGI3_HUMAN antibody
    • Membrane Associated Guanylate kinase Related 3 antibody
    • Membrane-associated guanylate kinase antibody
    • Membrane-associated guanylate kinase inverted 3 antibody
    • RP4-730K3.1 antibody
    • SLIPR antibody
    • WW and PDZ domain-containing protein 3 antibody
    see all

Anti-MAGI3 antibody 图像

  • All lanes : Anti-MAGI3 antibody (ab118615) at 1/500 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal liver lysate


    Predicted band size : 123, 160 kDa
    Observed band size : 130,160 kDa (why is the actual band size different from the predicted?)
  • ab118615 at 1/100 dilution, staining MAGI3 in formalin fixed, paraffin embedded Human fetal testis tissue by Immunohistochemistry. Note membrane and nuclear staining.

Anti-MAGI3 antibody (ab118615)参考文献

ab118615 has not yet been referenced specifically in any publications.

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