Anti-MAGI3抗体(ab111692)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to MAGI3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 112-342 of Human MAGI3, isoform 1~4 (AAI30410).

  • 阳性对照
    • IMR32 whole cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 123 kDa.

靶标

  • 功能
    Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 6 PDZ (DHR) domains.
    Contains 2 WW domains.
  • 翻译后修饰
    Ubiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex.
  • 细胞定位
    Cell membrane. Cell junction > tight junction. Nucleus. Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ730K3.2 antibody
    • MAGI-3 antibody
    • magi3 antibody
    • MAGI3_HUMAN antibody
    • Membrane Associated Guanylate kinase Related 3 antibody
    • Membrane associated guanylate kinase, WW and PDZ domain containing 3 antibody
    • Membrane associated guanylate kinase, WW and PDZ domain containing protein 3 antibody
    • Membrane-associated guanylate kinase antibody
    • Membrane-associated guanylate kinase inverted 3 antibody
    • RP4-730K3.1 antibody
    • SLIPR antibody
    • WW and PDZ domain-containing protein 3 antibody
    see all

图片

  • Anti-MAGI3 antibody (ab111692) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size : 123 kDa

实验方案

文献

ab111692 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your reply and all the additional information. This makes the problems you have been experiencing much clearer to understand. It is clear that you have spent a lot of time in trying to optimise these antibodies and I am sorry that they ar...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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