概述

  • 产品名称
  • 描述
    兔多克隆抗体to MAGEE2
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: EALKFVSDAH DEEPWSWPEE YNKALEGDKT KERSLTAGLE FWSEDTMNDK ANDLVQLAIS VTEEMLPIHQ DELLAHTGKE FEDVFPNILN RATLILDMFY GLSLIEVDT corresponding to amino acids of Human MAGEE2.

  • 阳性对照
    • Human colon tissue; MAGEE2 over-expression HEK293T lysate.

性能

应用

Our Abpromise guarantee covers the use of ab122118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 序列相似性
    Contains 2 MAGE domains.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HCA3 antibody
    • Hepatocellular carcinoma-associated protein 3 antibody
    • Hepatocellular carcinoma-associated protein HCA32 antibody
    • MAGE-E2 antigen antibody
    • MAGE2_HUMAN antibody
    • MAGEE2 antibody
    • melanoma antigen family E, 2 antibody
    • Melanoma-associated antigen E2 antibody
    • MGC138154 antibody
    • OTTHUMP00000023584 antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and nucleoli. Recommended concentration of ab122118 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-MAGEE2 antibody (ab122118) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    Developed using the ECL technique
  • ab122118, at 1/50 dilution, staining MAGEE2 in paraffin embedded Human colon tissue by Immunohistochemistry.

文献

ab122118 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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