概述

  • 产品名称Anti-MAGEA4抗体
    参阅全部 MAGEA4 一抗
  • 描述
    兔多克隆抗体to MAGEA4
  • 特异性While ab76177 has not been tested for cross-reactivity with other MAGE proteins, the immunogen shows >80% sequence homology with MAGEA1, MAGEA2, MAGEA3, MAGEB3, MAGEA6, MAGEA8, MAGEA9, MAGEA10, and MAGEA12. Based on this information, ab76177 may cross-react with these proteins.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    synthetic peptide from the C-terminus of Human MAGEA4

  • 阳性对照
    • HepG2 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab76177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
  • 组织特异性Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
  • 序列相似性Contains 1 MAGE domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cancer/testis antigen 1.4 antibody
    • CT1.4 antibody
    • MAGA4_HUMAN antibody
    • MAGE 4 antibody
    • MAGE 4 antigen antibody
    • MAGE 41 antibody
    • MAGE 41 antigen antibody
    • MAGE 4A antibody
    • MAGE 4B antibody
    • MAGE X2 antibody
    • MAGE X2 antigen antibody
    • MAGE-4 antigen antibody
    • MAGE-41 antigen antibody
    • MAGE-X2 antigen antibody
    • MAGE4 antibody
    • MAGE41 antibody
    • MAGE4A antibody
    • MAGE4B antibody
    • MAGEA 4 antibody
    • MAGEA4 antibody
    • Melanoma antigen family A 4 antibody
    • Melanoma associated antigen 4 antibody
    • Melanoma-associated antigen 4 antibody
    • MGC21336 antibody
    see all

Anti-MAGEA4 antibody 图像

  • Anti-MAGEA4 antibody (ab76177) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

Anti-MAGEA4 antibody (ab76177)参考文献

This product has been referenced in:
  • Altman E  et al. Characterization of human spermatogonial stem cell markers in fetal, pediatric, and adult testicular tissues. Reproduction 148:417-27 (2014). Read more (PubMed: 25030892) »

See 1 Publication for this product

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