概述

  • 产品名称
  • 描述
    兔多克隆抗体to MAG1
  • 特异性
    Detects endogenous levels of total PLCH protein.
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human MAG1 aa 381-430 (C terminal).
    Database link: Q53EU6

  • 阳性对照
    • Extracts from Jurkat, HeLa, and COLO205 cells. HepG2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab64979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ELISA 1/1000.
ICC/IF 1/100 - 1/500.

靶标

  • 功能
    Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Overexpression activates the mTOR pathway.
  • 组织特异性
    Widely expressed. Expressed in liver, kidney, testis, brain, heart, skeletal muscle, thyroid, prostate, thymus and placenta. Also expressed lung and adipose tissue.
  • 通路
    Glycerolipid metabolism; triacylglycerol biosynthesis.
    Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
  • 序列相似性
    Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
  • 结构域
    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 acylglycerol 3 phosphate O acyltransferase 8 antibody
    • 1 acylglycerol 3 phosphate O acyltransferase 9 antibody
    • 1 AGP acyltransferase 9 antibody
    • 1 AGPAT 9 antibody
    • 1-acylglycerol-3-phosphate O-acyltransferase 9 antibody
    • 1-AGP acyltransferase 9 antibody
    • 1-AGPAT 9 antibody
    • Acyl CoA:glycerol 3 phosphate acyltransferase 3 antibody
    • Acyl-CoA:glycerol-3-phosphate acyltransferase 3 antibody
    • AGPAT8 antibody
    • AGPAT9 antibody
    • Endoplasmic reticulum associated GPAT antibody
    • Glycerol-3-phosphate acyltransferase 3 antibody
    • GPAT-3 antibody
    • GPAT3 antibody
    • GPAT3_HUMAN antibody
    • hGPAT3 antibody
    • HMFN0839 antibody
    • LPAAT theta antibody
    • LPAAT-theta antibody
    • Lung cancer metastasis associated protein antibody
    • Lung cancer metastasis-associated protein 1 antibody
    • Lysophosphatidic acid acyltransferase theta antibody
    • MAG 1 antibody
    • MAG-1 antibody
    • MGC11324 antibody
    • PLCH antibody
    see all

Anti-MAG1 antibody 图像

  • This image shows HepG2 cells stained with ab64979 at 1/100 -1/500 dilution; with and without the immunising peptide.

  • All lanes : Anti-MAG1 antibody (ab64979) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells (5-30µg total protein)
    Lane 2 : extracts from HeLa cells (5-30µg total protein)
    Lane 3 : extracts from COLO205 cells (5-30µg total protein)
    Lane 4 : extracts from COLO205 cells (5-30µg total protein) and 5-10µg of the immunising peptide


    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 117 kDa,22 kDa. We are unsure as to the identity of these extra bands.

Anti-MAG1 antibody (ab64979)参考文献

ab64979 has not yet been referenced specifically in any publications.

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