The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Several other bands are also visible; we have no explanation for their presence.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Overexpression activates the mTOR pathway.
组织特异性Widely expressed. Expressed in liver, kidney, testis, brain, heart, skeletal muscle, thyroid, prostate, thymus and placenta. Also expressed lung and adipose tissue.
This image shows HepG2 cells stained with ab64979 at 1/500 -1/1000 dilution; with and without the immunising peptide.
Western blot - MAG1 antibody (ab64979)
All lanes : Anti-MAG1 antibody (ab64979) at 1/500 dilution
Lane 1 : extracts from Jurkat cells (5-30µg total protein) Lane 2 : extracts from HeLa cells (5-30µg total protein) Lane 3 : extracts from COLO205 cells (5-30µg total protein) Lane 4 : extracts from COLO205 cells (5-30µg total protein) and 5-10µg of the immunising peptide
Predicted band size : 49 kDa Observed band size : 49 kDa Additional bands at : 117 kDa,22 kDa. We are unsure as to the identity of these extra bands.
Anti-MAG1 antibody (ab64979)参考文献
has not yet been referenced specifically in any publications.