概述

  • 产品名称Anti-MAG1抗体
    参阅全部 MAG1 一抗
  • 描述
    兔多克隆抗体to MAG1
  • 特异性Detects endogenous levels of total PLCH protein.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized peptide derived from C-terminal of human PLCH.

  • 阳性对照
    • Extracts from Jurkat, HeLa, and COLO205 cells. HepG2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab64979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
ICC/IF
  • 应用说明ELISA: 1/5000.
    ICC/IF: 1/500 - 1/1000.
    WB: 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Several other bands are also visible; we have no explanation for their presence.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Overexpression activates the mTOR pathway.
    • 组织特异性Widely expressed. Expressed in liver, kidney, testis, brain, heart, skeletal muscle, thyroid, prostate, thymus and placenta. Also expressed lung and adipose tissue.
    • 通路Glycerolipid metabolism; triacylglycerol biosynthesis.
      Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
    • 序列相似性Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
    • 结构域The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
    • 细胞定位Endoplasmic reticulum membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1 acylglycerol 3 phosphate O acyltransferase 8 antibody
      • 1 acylglycerol 3 phosphate O acyltransferase 9 antibody
      • 1 AGP acyltransferase 9 antibody
      • 1 AGPAT 9 antibody
      • 1-acylglycerol-3-phosphate O-acyltransferase 9 antibody
      • 1-AGP acyltransferase 9 antibody
      • 1-AGPAT 9 antibody
      • Acyl CoA:glycerol 3 phosphate acyltransferase 3 antibody
      • Acyl-CoA:glycerol-3-phosphate acyltransferase 3 antibody
      • AGPAT8 antibody
      • AGPAT9 antibody
      • Endoplasmic reticulum associated GPAT antibody
      • Glycerol-3-phosphate acyltransferase 3 antibody
      • GPAT-3 antibody
      • GPAT3 antibody
      • GPAT3_HUMAN antibody
      • hGPAT3 antibody
      • HMFN0839 antibody
      • LPAAT theta antibody
      • LPAAT-theta antibody
      • Lung cancer metastasis associated protein antibody
      • Lung cancer metastasis-associated protein 1 antibody
      • Lysophosphatidic acid acyltransferase theta antibody
      • MAG 1 antibody
      • MAG-1 antibody
      • MGC11324 antibody
      • PLCH antibody
      see all

    Anti-MAG1 antibody 图像

    • This image shows HepG2 cells stained with ab64979 at 1/500 -1/1000 dilution; with and without the immunising peptide.
    • All lanes : Anti-MAG1 antibody (ab64979) at 1/500 dilution

      Lane 1 : extracts from Jurkat cells (5-30µg total protein)
      Lane 2 : extracts from HeLa cells (5-30µg total protein)
      Lane 3 : extracts from COLO205 cells (5-30µg total protein)
      Lane 4 : extracts from COLO205 cells (5-30µg total protein) and 5-10µg of the immunising peptide


      Predicted band size : 49 kDa
      Observed band size : 49 kDa
      Additional bands at : 117 kDa,22 kDa. We are unsure as to the identity of these extra bands.

    Anti-MAG1 antibody (ab64979)参考文献

    ab64979 has not yet been referenced specifically in any publications.

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