重组Anti-MAG/GMA抗体[EP971Y] (ab46803)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP971Y] to MAG/GMA
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MAG/GMA抗体[EP971Y]
参阅全部 MAG/GMA 一抗 -
描述
兔单克隆抗体[EP971Y] to MAG/GMA -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human MAG/GMA aa 50-150.
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阳性对照
- WB: Human, Mouse and Rat brain lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP971Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46803于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa).
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说明 |
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WB
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa). |
靶标
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功能
Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid. -
序列相似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4099 Human
- Entrez Gene: 17136 Mouse
- Entrez Gene: 29409 Rat
- Omim: 159460 Human
- SwissProt: P20916 Human
- SwissProt: P20917 Mouse
- SwissProt: P07722 Rat
- Unigene: 643440 Human
see all -
别名
- GMA antibody
- MAG antibody
- MAG_HUMAN antibody
see all
图片
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All lanes : Anti-MAG/GMA antibody [EP971Y] (ab46803) at 1/10000 dilution
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting dilution: 5% NFDM/TBST
Exposure time:
Lane1: 180 seconds
Lane2: 80 seconds
Lane3: 60 seconds
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab46803 被引用在 1 文献中.
- Zou S et al. Oligodendrocytes Are Targets of HIV-1 Tat: NMDA and AMPA Receptor-Mediated Effects on Survival and Development. J Neurosci 35:11384-98 (2015). PubMed: 26269645