概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to MAG
  • 经测试应用
    适用于: ICC/IF, Flow Cyt, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Cow, Cat, Human, Monkey
    预测可用于: Orangutan
  • 免疫原

    Recombinant fragment: GDLGGYNQYT FSEHSVLDIV NTPNIVVPPE VVAGTEVEVS CMVPDNCPEL RPELSWLGHE GLGEPAVLGR LREDEGTWVQ VSLLHFVPTR, corresponding to amino acids 119-208 of Human MAG (NP_002352) with a proprietary tag.

  • 阳性对照
    • Partial tagged recombinant Human MAG protein (Immunogen), Jurkat cell lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: SKNSH.

性能

应用

Our Abpromise guarantee covers the use of ab89780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/400.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/2000 - 1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 63 kDa. Isoform a, 69 kDa; Isoform b, 63 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid.
  • 序列相似性
    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GMA antibody
    • MAG antibody
    • MAG_HUMAN antibody
    • Myelin associated glycoprotein antibody
    • Myelin-associated glycoprotein antibody
    • S MAG antibody
    • S-MAG antibody
    • Sialic acid binding Ig like lectin 4A antibody
    • Sialic acid binding immunoglobulin like lectin 4A antibody
    • Siglec 4a antibody
    • Siglec-4a antibody
    • SIGLEC4A antibody
    • SPG75 antibody
    see all

Anti-MAG antibody 图像

  • Anti-MAG antibody (ab89780) at 5 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 63 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  • ab89780 staining MAG in lamb spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/5000 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab89780 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89780, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min) used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • ab89780 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89780 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab89780 staining MAG in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with acetone, permeablized with methanol and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100) at 4°C for 18 hours. An Alexa Fluor® 594-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • IHC-P image of MAG staining on cow peripheral nerve sections using ab89780 (1:4000). The sections were fixed in Formaldehyde and subjected to heat mediated antigen retrieval using citric acid (pH 6). The sections were blocked using 1% BSA for 10 mins at 21°C.

    See Abreview

  • Anti-MAG antibody (ab89780) at 5 µg/ml + Partial tagged recombinant Human MAG protein at 0.2 µg

    Predicted band size : 63 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 36 kDa.

Anti-MAG antibody (ab89780)参考文献

This product has been referenced in:
  • Rubovitch V  et al. Restoring GM1 ganglioside expression ameliorates axonal outgrowth inhibition and cognitive impairments induced by blast traumatic brain injury. Sci Rep 7:41269 (2017). Read more (PubMed: 28112258) »
  • Dohare P  et al. AMPA-Kainate Receptor Inhibition Promotes Neurologic Recovery in Premature Rabbits with Intraventricular Hemorrhage. J Neurosci 36:3363-77 (2016). Read more (PubMed: 26985043) »

See all 14 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Sciatic nerve)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
Sciatic nerve
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 19 2016

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Sciatic nerve)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
Sciatic nerve
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 19 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Sheep Tissue sections (Lamb spinal cord)
Specification
Lamb spinal cord
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 03 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
Yes - methanol
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Sep 12 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (10% Gel)
Sample
Rat Tissue lysate - whole (cerebral cortex)
Specification
cerebral cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Sep 11 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Lizard Tissue sections (Spinal cord)
Specification
Spinal cord
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Jun 18 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Sciatic nerve)
Loading amount
30 µg
Specification
Sciatic nerve
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

提交于 May 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (brain)
Specification
brain
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

提交于 Nov 06 2012

Thank you for contacting us.

We do not know whether this antibody will recognize S- and/or L-MAG as this has not been specifically tested in our lab. I don't know much about the differences between S- and L-MAG, but in theory if they contain...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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