概述

性能

应用

Our Abpromise guarantee covers the use of ab82837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Use at an assay dependent dilution.
    Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/20000 is determined for one of the three peptides.
    WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
    • 组织特异性Ubiquitous.
    • 疾病相关Defects in MAFB are the cause of multicentric carpotarsal osteolysis syndrome (MCTO) [MIM:166300]. MCTO is a rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients.
    • 序列相似性Belongs to the bZIP family. Maf subfamily.
      Contains 1 bZIP domain.
    • 结构域The leucine-zipper domain is involved in the interaction with LRPICD.
    • 翻译后修饰Phosphorylated by GSK3 and MAPK13 on serine and threonine residues.
      Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Kreisler antibody
      • Kreisler (mouse) maf related leucine zipper homolog antibody
      • Kreisler maf related leucine zipper homolog antibody
      • KRML antibody
      • Maf-B antibody
      • Mafb antibody
      • MAFB/Kreisler basic region/leucine zipper transcription factor antibody
      • MAFB_HUMAN antibody
      • MGC43127 antibody
      • Segmentation protein KR antibody
      • Transcription factor MafB antibody
      • V maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) antibody
      • V-maf musculoaponeurotic fibrosarcoma oncogene homolog B antibody
      see all

    Anti-MafB antibody 图像

    • All lanes : Anti-MafB antibody (ab82837) at 1/500 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    Anti-MafB antibody (ab82837)参考文献

    ab82837 has not yet been referenced specifically in any publications.

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