概述

性能

应用

Our Abpromise guarantee covers the use of ab66506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 20436469
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Can be blocked with MafB peptide (ab205727). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
Flow Cyt Use 4µg for 106 cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in MAFB are the cause of multicentric carpotarsal osteolysis syndrome (MCTO) [MIM:166300]. MCTO is a rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients.
  • 序列相似性Belongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • 结构域The leucine-zipper domain is involved in the interaction with LRPICD.
  • 翻译后修饰Phosphorylated by GSK3 and MAPK13 on serine and threonine residues.
    Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Kreisler antibody
    • Kreisler (mouse) maf related leucine zipper homolog antibody
    • Kreisler maf related leucine zipper homolog antibody
    • KRML antibody
    • Maf-B antibody
    • Mafb antibody
    • MAFB/Kreisler basic region/leucine zipper transcription factor antibody
    • MAFB_HUMAN antibody
    • MGC43127 antibody
    • Segmentation protein KR antibody
    • Transcription factor MafB antibody
    • V maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) antibody
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog B antibody
    see all

Anti-MafB antibody 图像

  • Anti-MafB antibody (ab66506) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analyisis of human kidney tissue labelling MafB with ab66506 at an 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • ab66506, at 4 µg/cells, staining MAFB in mouse bone marrow by flow cytometry. Cells were paraformaldehyde fixed and permeabilized using Saponin. The secondary antibody was ab6717, a FITC-conjugated goat polyclonal to rabbit IgG.

    See Abreview

Anti-MafB antibody (ab66506)参考文献

This product has been referenced in:
  • Bunaciu RP  et al. 6-Formylindolo(3,2-b)Carbazole (FICZ) Modulates the Signalsome Responsible for RA-Induced Differentiation of HL-60 Myeloblastic Leukemia Cells. PLoS One 10:e0135668 (2015). WB ; Human . Read more (PubMed: 26287494) »
  • Kilcoyne KR  et al. Fetal programming of adult Leydig cell function by androgenic effects on stem/progenitor cells. Proc Natl Acad Sci U S A 111:E1924-32 (2014). IHC-P . Read more (PubMed: 24753613) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Bone marrow)
Specification Bone marrow
Fixative Paraformaldehyde
Permeabilization Yes - Saponin
Username

Dr. Kay Muthu

Verified customer

提交于 Jul 16 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Bone marrow)
Specification Bone marrow
Preparation Cell harvesting/tissue preparation method: Magnetic cell sorting
Sample buffer: IMDM
Fixation Paraformaldehyde
Permeabilization Yes - Saponin
Gating Strategy live cells
Username

Dr. Kay Muthu

Verified customer

提交于 Jul 15 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"