概述

  • 产品名称
  • 描述
    兔多克隆抗体to MafB
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Chicken, Non human primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 MafB.

    (Peptide available as ab71662.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell, HepG2 Whole Cell, Caco 2 Whole Cell, Rat Thymus Tissue (data not shown), Rat Colon Tissue (data not shown)

性能

应用

Our Abpromise guarantee covers the use of ab65953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
  • 组织特异性
    Ubiquitous.
  • 疾病相关
    Defects in MAFB are the cause of multicentric carpotarsal osteolysis syndrome (MCTO) [MIM:166300]. MCTO is a rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients.
  • 序列相似性
    Belongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • 结构域
    The leucine-zipper domain is involved in the interaction with LRPICD.
  • 翻译后修饰
    Phosphorylated by GSK3 and MAPK13 on serine and threonine residues.
    Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Kreisler antibody
    • Kreisler (mouse) maf related leucine zipper homolog antibody
    • Kreisler maf related leucine zipper homolog antibody
    • KRML antibody
    • MAF bZIP transcription factor B antibody
    • Maf-B antibody
    • Mafb antibody
    • MAFB/Kreisler basic region/leucine zipper transcription factor antibody
    • MAFB_HUMAN antibody
    • MGC43127 antibody
    • Segmentation protein KR antibody
    • Transcription factor MafB antibody
    • V maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) antibody
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog B antibody
    see all

图片

  • All lanes : Anti-MafB antibody (ab65953) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,55 kDa,63 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab65953 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65953, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 cells at 5µg/ml.

文献

This product has been referenced in:
  • Fujiwara T  et al. RNA-binding protein Musashi2 induced by RANKL is critical for osteoclast survival. Cell Death Dis 7:e2300 (2016). WB ; Mouse . Read more (PubMed: 27441652) »

See 1 Publication for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab65953.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册