Anti-MafA抗体(ab17976)
Key features and details
- Rabbit polyclonal to MafA
- Suitable for: WB, EMSA
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MafA抗体
参阅全部 MafA 一抗 -
描述
兔多克隆抗体to MafA -
宿主
Rabbit -
经测试应用
适用于: WB, EMSAmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide corresponding to Mouse MafA (C terminal).
Database link: Q8CF90 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17976于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000.
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EMSA |
Use a concentration of 0.05 - 0.25 mg/ml.
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说明 |
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WB
1/1000 - 1/10000. |
EMSA
Use a concentration of 0.05 - 0.25 mg/ml. |
靶标
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功能
Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. -
序列相似性
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain. -
翻译后修饰
Ubiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway. -
细胞定位
Nucleus. Detected in nuclei of pancreas islet beta cells. - Information by UniProt
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数据库链接
- Entrez Gene: 389692 Human
- Entrez Gene: 366949 Rat
- Omim: 610303 Human
- SwissProt: Q8NHW3 Human
- Unigene: 521914 Human
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别名
- HMafA antibody
- Mafa antibody
- MAFA_HUMAN antibody
see all
图片
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Detection of MafA, MafB and cMaf by Western Blot. Samples: Nuclear extract (6 µg) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody, anti-MafB Antibody or anti-c-Maf antibody. Each antibody was used at 0.5 µg/ml. Detection: Chemiluminescence with a 5 second exposure.
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Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069, anti-MafB Antibody or anti-c-Maf antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab17976 被引用在 5 文献中.
- Xu J et al. MAFB mediates the therapeutic effect of sleeve gastrectomy for obese diabetes mellitus by activation of FXR expression. Braz J Med Biol Res 51:e7312 (2018). PubMed: 29846411
- Roth Flach RJ et al. Protein Kinase Mitogen-activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Promotes Obesity-induced Hyperinsulinemia. J Biol Chem 291:16221-30 (2016). IHC-P ; Mouse . PubMed: 27226575
- Sakano D et al. VMAT2 identified as a regulator of late-stage ß-cell differentiation. Nat Chem Biol 10:141-8 (2014). PubMed: 24316738
- Hunter KE et al. Identification and characterization of poorly differentiated invasive carcinomas in a mouse model of pancreatic neuroendocrine tumorigenesis. PLoS One 8:e64472 (2013). WB, IHC-P ; Mouse . PubMed: 23691228
- Vargas N et al. TAT-Mediated Transduction of MafA Protein In Utero Results in Enhanced Pancreatic Insulin Expression and Changes in Islet Morphology. PLoS One 6:e22364 (2011). WB ; Mouse . PubMed: 21857924