概述

  • 产品名称
  • 描述
    兔多克隆抗体to MAFA
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    3 synthetic peptides (Human) conjugated to KLH

  • 阳性对照
    • Recombinant protein produced in an induced culture of E.coli.

性能

应用

Our Abpromise guarantee covers the use of ab86020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 37 kDa.

靶标

  • 功能
    Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
  • 序列相似性
    Belongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • 翻译后修饰
    Ubiquitinated, leading to its degradation by the proteasome.
    Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
  • 细胞定位
    Nucleus. Detected in nuclei of pancreas islet beta cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HMafA antibody
    • Mafa antibody
    • MAFA_HUMAN antibody
    • Pancreatic beta cell specific transcriptional activator antibody
    • Pancreatic beta-cell-specific transcriptional activator antibody
    • RIPE3b1 antibody
    • Transcription factor MafA antibody
    • Transcription factor RIPE3b1 antibody
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian) antibody
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
    see all

图片

  • All lanes : Anti-MAFA antibody (ab86020) at 1/1000 dilution

    Lane 1 : Uninduced (negative control) culture of E.coli
    Lane 2 : Induced culture of E.coli

    Secondary
    anti-Rabbit IgG HRP conjugated at 1/1000 dilution

    Predicted band size : 37 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab86020 has not yet been referenced specifically in any publications.

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