Anti-Mael抗体(ab106713)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to Mael
  • 经测试应用
    适用于: WB, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 16 amino acid peptide near the C terminus of Human Mael (NP_065199).

  • 阳性对照
    • Human skeletal muscle tissue; HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab106713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa.
ICC Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

靶标

  • 功能
    Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for localization of germ-cell factors to the meiotic nuage.
  • 组织特异性
    Testis-specific. Expressed in various cancer cell lines, probably due to demethylation of its promoter.
  • 序列相似性
    Belongs to the maelstrom family.
    Contains 1 HMG box DNA-binding domain.
  • 细胞定位
    Cytoplasm. Nucleus. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cancer/testis antigen 128 antibody
    • CT128 antibody
    • FLJ14904 antibody
    • mael antibody
    • MAEL_HUMAN antibody
    • maelstrom homolog (Drosophila) antibody
    • Maelstrom homolog antibody
    • OTTHUMP00000032344 antibody
    • Protein maelstrom homolog antibody
    • RP11-102C16.1 antibody
    see all

图片

  • Immunofluorescence of Mael in HeLa cells with ab106713 at 20 ug/mL.

  • Anti-Mael antibody (ab106713) at 1 µg/ml + HeLa cell lysate at 15 µg

    Predicted band size : 49 kDa
  • ab106713, at 5 µg/ml, staining in Hela cells with Maelstrom by Immunocytochemistry.

文献

ab106713 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample
Marmoset (common) Tissue sections (Adult testis)
Specification
Adult testis
Permeabilization
No
Fixative
Formaldehyde
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提交于 Oct 01 2013

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