Anti-MAdCAM1抗体[MECA-367] (FITC) (ab24941)

概述

  • 产品名称Anti-MAdCAM1抗体[MECA-367] (FITC)
    参阅全部 MAdCAM1 一抗
  • 描述
    大鼠单克隆抗体[MECA-367] to MAdCAM1 (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 经测试应用适用于: Blocking, Flow Cyt, IHC-Fr, IP, WB, Othermore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation: Endothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes.

  • 表位This antibody reacts with an epitope located in the first domain of MAdCAM1.

性能

应用

Our Abpromise guarantee covers the use of ab24941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Blocking
Flow Cyt
IHC-Fr
IP
WB
AP
Other
  • 应用说明AP: Use at an assay dependent dilution.
    BL: Use at an assay dependent dilution.
    Note: This antibody is useful for in vitro cellular binding blocking, and for blocking lymphocyte homing to Peyers patch HEV in vivo.
    Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at a concentration of 1 µg/ml.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 44 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
    • 组织特异性Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
    • 序列相似性Contains 2 Ig-like (immunoglobulin-like) domains.
    • 翻译后修饰The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Addressin mucosal antibody
      • hMAdCAM 1 antibody
      • hMAdCAM-1 antibody
      • MACAM1 antibody
      • MADCA_HUMAN antibody
      • MAdCAM 1 antibody
      • MAdCAM-1 antibody
      • Madcam1 antibody
      • Mucosal addressin cell adhesion molecule 1 antibody
      • Mucosal vascular addressin cell adhesion molecule 1 antibody
      see all

    Anti-MAdCAM1 antibody [MECA-367] (FITC) (ab24941)参考文献

    This product has been referenced in:
    • Hwang IY  et al. The loss of Gnai2 and Gnai3 in B cells eliminates B lymphocyte compartments and leads to a hyper-IgM like syndrome. PLoS One 8:e72596 (2013). IHC-Fr ; Mouse . Read more (PubMed: 23977324) »

    See 1 Publication for this product

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