Anti-MAdCAM1抗体[AP-MAB0842] (ab80680)

概述

  • 产品名称Anti-MAdCAM1抗体[AP-MAB0842]
    参阅全部 MAdCAM1 一抗
  • 描述
    大鼠单克隆抗体[AP-MAB0842] to MAdCAM1
  • 经测试应用适用于: IHC-P, Inhibition Assay, IP, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Mouse Endothelial cells

  • 常规说明
    Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).


    ab80680 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
    ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases.

性能

应用

Our Abpromise guarantee covers the use of ab80680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Inhibition Assay Use at an assay dependent concentration.

ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor.

IP 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
Flow Cyt 1/200 - 1/400.

靶标

  • 功能Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
  • 组织特异性Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
  • 序列相似性Contains 2 Ig-like (immunoglobulin-like) domains.
  • 翻译后修饰The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Addressin mucosal antibody
    • hMAdCAM 1 antibody
    • hMAdCAM-1 antibody
    • MACAM1 antibody
    • MADCA_HUMAN antibody
    • MAdCAM 1 antibody
    • MAdCAM-1 antibody
    • Madcam1 antibody
    • Mucosal addressin cell adhesion molecule 1 antibody
    • Mucosal vascular addressin cell adhesion molecule 1 antibody
    see all

Anti-MAdCAM1 antibody [AP-MAB0842] 图像

  • ab80680 staining MAdCAM1 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate, pH 6.0. Samples were incubated with primary antibody (1/100 in TBS + 1% BSA) for 30 minutes. An undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-MAdCAM1 antibody [AP-MAB0842] (ab80680)参考文献

ab80680 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step (agent) for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Sample Mouse Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde
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Mr. Alex Van Engelenburg

Verified customer

提交于 Jul 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"