Anti-MAdCAM1抗体[AP-MAB0842] (ab80680)
Key features and details
- Rat monoclonal [AP-MAB0842] to MAdCAM1
- Suitable for: IHC-Fr, IP, IHC-P, Flow Cyt, Inhibition Assay
- Reacts with: Mouse
- Isotype: IgG2
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MAdCAM1抗体[AP-MAB0842]
参阅全部 MAdCAM1 一抗 -
描述
大鼠单克隆抗体[AP-MAB0842] to MAdCAM1 -
宿主
Rat -
经测试应用
适用于: IHC-Fr, IP, IHC-P, Flow Cyt, Inhibition Assaymore details -
种属反应性
与反应: Mouse -
免疫原
Mouse Endothelial cells
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常规说明
Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).ab80680 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
The IgG fraction of culture supernatant was purified by Protein A affinity chromatography. -
Primary antibody说明
ab80680 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases. -
克隆
单克隆 -
克隆编号
AP-MAB0842 -
同种型
IgG2 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80680于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
|
Flow Cyt |
Use at an assay dependent concentration.
|
|
Inhibition Assay |
Use at an assay dependent concentration.
ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor. |
说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Inhibition Assay
Use at an assay dependent concentration. ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor. |
靶标
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功能
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. -
组织特异性
Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes. -
序列相似性
Contains 2 Ig-like (immunoglobulin-like) domains. -
翻译后修饰
The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 17123 Mouse
- SwissProt: Q61826 Mouse
- Unigene: 391556 Mouse
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别名
- Addressin mucosal antibody
- hMAdCAM 1 antibody
- hMAdCAM-1 antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab80680 被引用在 1 文献中.
- Gao RY et al. Bile acids modulate colonic MAdCAM-1 expression in a murine model of combined cholestasis and colitis. Mucosal Immunol 14:479-490 (2021). PubMed: 33004979