概述

  • 产品名称Anti-MAdCAM1抗体[314G8]
    参阅全部 MAdCAM1 一抗
  • 描述
    小鼠单克隆抗体[314G8] to MAdCAM1
  • 经测试应用适用于: Flow Cyt, IHC-P, ELISA, WB, Functional Studies, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant soluble MAdCAM-1-fc fusion protein (Human).

  • 常规说明


    Clone 314G8 reacts with the ligand binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM1 with alpha 4 beta 7 (see reference 1).

性能

应用

Our Abpromise guarantee covers the use of ab34247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 43 kDa).
Functional Studies Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
  • 组织特异性Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
  • 序列相似性Contains 2 Ig-like (immunoglobulin-like) domains.
  • 翻译后修饰The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Addressin mucosal antibody
    • hMAdCAM 1 antibody
    • hMAdCAM-1 antibody
    • MACAM1 antibody
    • MADCA_HUMAN antibody
    • MAdCAM 1 antibody
    • MAdCAM-1 antibody
    • Madcam1 antibody
    • Mucosal addressin cell adhesion molecule 1 antibody
    • Mucosal vascular addressin cell adhesion molecule 1 antibody
    see all

Anti-MAdCAM1 antibody [314G8] (ab34247)参考文献

This product has been referenced in:
  • Allavena R  et al. CNS elevation of vascular and not mucosal addressin cell adhesion molecules in patients with multiple sclerosis. Am J Pathol 176:556-62 (2010). IHC-Fr ; Human . Read more (PubMed: 20035048) »

See 1 Publication for this product

Product Wall

The following paper describes production of the clone and characterization in three of the assays we list on the datasheet: Functional Assays, IHC-P, and WB

http://www.nature.com/icb/journal/v82/n4/full/icb200462a.html

Leung, E. e...

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Thank you for contacting us.



I am sorry to hear you are experiencing difficulties with ab34143 in IHC-Fr. This antibody is garanteed to work on Human samplesin IHC-Fr, Flow Cyt, ELISA, IHC-P andWB.

We take product complai...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"