概述

  • 产品名称
  • 描述
    兔多克隆抗体to MAD4
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog
  • 免疫原

    A region within synthetic peptide: DFAREKTKAA GLVRKAPNNR SSHNELEKHR RAKLRLYLEQ LKQLVPLGPD, corresponding to N terminal amino acids 36-85 of Human MAD4

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab66367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 - 2.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 功能
    Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
  • 序列相似性
    Contains 1 bHLH (basic helix-loop-helix) domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHc12 antibody
    • Class C basic helix loop helix protein 12 antibody
    • Class C basic helix-loop-helix protein 12 antibody
    • MAD 4 antibody
    • MAD4 antibody
    • Mad4 homolog antibody
    • MAD4_HUMAN antibody
    • Max associated protein 4 antibody
    • MAX dimerization protein 4 antibody
    • Max dimerizer 4 antibody
    • Max interacting transcriptional repressor MAD4 antibody
    • Max-associated protein 4 antibody
    • Max-interacting transcriptional repressor MAD4 antibody
    • MST 149 antibody
    • MST149 antibody
    • MSTP 149 antibody
    • MSTP149 antibody
    • MXD 4 antibody
    • MXD4 antibody
    see all

图片

  • All lanes : Anti-MAD4 antibody (ab66367) at 0.25 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : molecular marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 23 kDa
    Observed band size : 30,48 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab66367 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (foreskin)
Permeabilization
No
Specification
foreskin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
none
Username

Mr. Julien Coutier

Verified customer

提交于 Jul 05 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Primary keratinocytes from skin epithelium)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Primary keratinocytes from skin epithelium
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 03 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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