Anti-MAD4抗体(ab111062)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to MAD4
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide: MELNSLLILL EAAEYLERR D REAEHGYA SV LPFDGDF ARE KTKAAG LVRK , corresponding to amino acids 1-50 of Human MAD4

  • 阳性对照
    • Human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab111062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
  • 序列相似性
    Contains 1 bHLH (basic helix-loop-helix) domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHc12 antibody
    • Class C basic helix loop helix protein 12 antibody
    • Class C basic helix-loop-helix protein 12 antibody
    • MAD 4 antibody
    • MAD4 antibody
    • Mad4 homolog antibody
    • MAD4_HUMAN antibody
    • Max associated protein 4 antibody
    • MAX dimerization protein 4 antibody
    • Max dimerizer 4 antibody
    • Max interacting transcriptional repressor MAD4 antibody
    • Max-associated protein 4 antibody
    • Max-interacting transcriptional repressor MAD4 antibody
    • MST 149 antibody
    • MST149 antibody
    • MSTP 149 antibody
    • MSTP149 antibody
    • MXD 4 antibody
    • MXD4 antibody
    see all

图片

  • ab111062 at 1/50 dilution staining MAD4 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

文献

ab111062 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Primary skin keratinocytes)
Permeabilization
Yes - Triton 0.1% 10 minutes
Specification
Primary skin keratinocytes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Mr. Julien Coutier

Verified customer

提交于 May 12 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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