概述
- 产品名称Anti-MAD3抗体[EPR3882]
参阅全部 MAD3 一抗 - 描述兔单克隆抗体[EPR3882] to MAD3
- 经测试应用适用于:
WB, IHC-P, Flow Cytmore details
不适用于: ICC or IP - 种属反应性与反应: Mouse, Rat, Human
- 免疫原
A synthetic peptide corresponding to residues in Human MAD3.
- 阳性对照
- HeLa and C6 cell lysates. Human brain, breast and testis tissue.
- 常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at -20ºC.
- 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- 纯度Tissue culture supernatant
- 克隆单克隆
- 克隆编号EPR3882
- 同种型IgG
- 研究领域
相关产品
- Positive Controls
应用
Our Abpromise guarantee covers the use of ab108525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/100 - 1/250. For antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. | |
| Flow Cyt | 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
- 功能Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
- 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
- 细胞定位Nucleus.
- Information by UniProt
-
数据库链接
- Entrez Gene: 83463 Human
- Entrez Gene: 17121 Mouse
- Entrez Gene: 252915 Rat
- Omim: 609450 Human
- SwissProt: Q9BW11 Human
- SwissProt: Q80US8 Mouse
- SwissProt: Q62912 Rat
- Unigene: 726161 Human
see all -
别名
- bHLHc13 antibody
- Class C basic helix-loop-helix protein 13 antibody
- FLJ35523 antibody
see all
Anti-MAD3 antibody [EPR3882] 图像
-
![Western blot - MAD3 antibody [EPR3882] (ab108525)](http://www.abcam.cn/ps/datasheet/images/108/ab108525/MAD3-Primary-antibodies-ab108525-1.JPG)
Western blot - MAD3 antibody [EPR3882] (ab108525)All lanes : Anti-MAD3 antibody [EPR3882] (ab108525) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 23 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAD3 antibody [EPR3882] (ab108525)](http://www.abcam.cn/ps/datasheet/images/108/ab108525/MAD3-Primary-antibodies-ab108525-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAD3 antibody [EPR3882] (ab108525)ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAD3 antibody [EPR3882] (ab108525)](http://www.abcam.cn/ps/datasheet/images/108/ab108525/MAD3-Primary-antibodies-ab108525-3.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAD3 antibody [EPR3882] (ab108525)ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAD3 antibody [EPR3882] (ab108525)](http://www.abcam.cn/ps/datasheet/images/108/ab108525/MAD3-Primary-antibodies-ab108525-4.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAD3 antibody [EPR3882] (ab108525)ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Anti-MAD3 antibody [EPR3882] (ab108525)参考文献
ab108525 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"