概述

  • 产品名称Anti-MAD3抗体
    参阅全部 MAD3 一抗
  • 描述
    兔多克隆抗体to MAD3
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: EPLASNIQVL LQAAEFLERR EREAEHGYAS LCPHRSPGPI HRRKKRPPQA , corresponding to N terminal amino acids 1-50 of Human MAD3

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

靶标

Anti-MAD3 antibody 图像

  • Anti-MAD3 antibody (ab80295) at 1 µg/ml + MAD3-transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 50000

    Predicted band size : 23 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa,55 kDa. We are unsure as to the identity of these extra bands.

Anti-MAD3 antibody (ab80295)参考文献

ab80295 has not yet been referenced specifically in any publications.

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