• 产品名称Anti-Mad2L2抗体
    参阅全部 Mad2L2 一抗
  • 描述
    兔多克隆抗体to Mad2L2
  • 特异性This antibody detects a single band just below the 21kD marker, that corresponds to Mad2L2 / Mad2B.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Xenopus laevis
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 3-14 of Human Mad2L2.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域



Our Abpromise guarantee covers the use of ab3630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明WB: 1/500. Detects a band of approximately ~20 kDa (predicted molecular weight: 24 kDa). Can be blocked with Mad2L2 (aa 3-14) peptide (human)

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
    • 组织特异性Ubiquitously expressed.
    • 序列相似性Contains 1 HORMA domain.
    • 细胞定位Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm.
    • Information by UniProt
    • 数据库链接
      • 别名
        • Homolog of REV7 S cerevisiae antibody
        • hREV7 antibody
        • MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
        • MAD2 homolog antibody
        • MAD2 like 2 antibody
        • MAD2 mitotic arrest deficient like 2 antibody
        • MAD2-like protein 2 antibody
        • MAD2B antibody
        • Mad2l2 antibody
        • MD2L2_HUMAN antibody
        • Mitotic Arrest Deficient 2 L2 antibody
        • Mitotic arrest deficient 2-like protein 2 antibody
        • Mitotic arrest deficient homolog like 2 antibody
        • Mitotic arrest deficient like 2 (yeast) antibody
        • Mitotic arrest deficient yeast homolog antibody
        • Mitotic spindle assembly checkpoint protein MAD2B antibody
        • Polymerase (DNA directed) zeta 2 accessory subunit antibody
        • POLZ2 antibody
        • REV 7 antibody
        • REV7 antibody
        • REV7 homolog antibody
        • Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody
        see all

      Anti-Mad2L2 antibody 图像

      • All lanes : Anti-Mad2L2 antibody (ab3630) at 1/500 dilution

        Lane 1 : Whole cell extract prepared from Hela cells
        Lane 2 : Nuclear lysates prepared from Hela cells

        Lysates/proteins at 20 µg per lane.

        Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

        Predicted band size : 24 kDa

      Anti-Mad2L2 antibody (ab3630)参考文献

      ab3630 has not yet been referenced specifically in any publications.

      Product Wall

      Abcam has not validated the combination of species/application used in this Abreview.
      Application Western blot
      Sample Zebrafish Tissue lysate - whole (whole larvae)
      Loading amount 100 µg
      Specification whole larvae
      Gel Running Conditions Reduced Denaturing
      Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

      Mr. Gregory Weber

      Verified customer

      提交于 Aug 04 2011