Anti-MAD2L1 binding protein抗体(ab77206)


  • 产品名称Anti-MAD2L1 binding protein抗体
    参阅全部 MAD2L1 binding protein 一抗
  • 描述
    小鼠单克隆抗体to MAD2L1 binding protein
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1 - 275 of Human MAD2L1 binding protein (AAH02904), with a 26 kDa tag

  • 阳性对照
    • HeLa nuclear extract; immunogen


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab77206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 31 kDa.
ELISA Use at an assay dependent dilution.


  • 功能May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
  • 序列相似性Belongs to the MAD2L1BP family.
  • 发展阶段During the cell cycle, levels increase and then remain constant until late mitosis after which they drop.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Cytoplasm > cytoskeleton > spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Caught by MAD2 protein antibody
    • CMT2 antibody
    • dJ261G23.1 antibody
    • KIAA0110 antibody
    • MAD2L1 binding protein antibody
    • MAD2L1 binding protein isoform 2 antibody
    • MAD2L1-binding protein antibody
    • Mad2l1bp antibody
    • MD2BP_HUMAN antibody
    • MGC11282 antibody
    • OTTHUMP00000016496 antibody
    • RP1 261G23.6 antibody
    see all

Anti-MAD2L1 binding protein antibody 图像

  • Anti-MAD2L1 binding protein antibody (ab77206) at 1/500 dilution + recombinant tagged MAD2L1 protein (the immunogen) at 0.2 µg

    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 31 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)
  • Anti-MAD2L1 binding protein antibody (ab77206) at 1/500 dilution + HeLa nuclear extract cell lysate at 25 µg

    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa

Anti-MAD2L1 binding protein antibody (ab77206)参考文献

ab77206 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.
I am not sure why the current vial of the antibody that you received was not as efficient as before. I am going to send you a new vial free of charge and hopefully that one should not have the same issues. Your new order ...

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Thank you for contacting Abcam.
We have not heard of any other similar issue regarding this product and it is a mouse monoclonal, so there should not be much, if any batch to batch variation. Also the storage and buffer conditions have not changed...

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