鸡多克隆抗体to MAD2L1 binding蛋白
Synthetic peptide, corresponding to N terminal amino acids 1-126 of MAD2L1 binding protein
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Predicted molecular weight: 31 kDa. We recommend using about 2.5 ug of total protein per lane in the colorimetric detection method, and about 0.25µg for ECL detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
Belongs to the MAD2L1BP family.
During the cell cycle, levels increase and then remain constant until late mitosis after which they drop.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Cytoplasm > cytoskeleton > spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
Information by UniProt
- Caught by MAD2 protein antibody
- CMT2 antibody
- dJ261G23.1 antibody
Western blot - Anti-MAD2L1 binding protein antibody (ab37584)
Anti-MAD2L1 binding protein antibody (ab37584) at 1/2000 dilution + E coli-derived fusion protein at 2.5 µgSecondary
Goat anti-IgY-HRP at 1/1000 dilutionPredicted band size:
31 kDaObserved band size:
80 kDa (why is the actual band size different from the predicted?
Predicted and actual molecular weights differ due to the use of a fusion protein.
has not yet been referenced specifically in any publications.
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