Anti-Mad2L1抗体(ab97777)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to Mad2L1
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 192 and 205 of Human Mad2L1 (NP_002349).

  • 阳性对照
    • NCIN87, U2OS, HepG2, MOLT4 and Raji cell lines; DLD-1 xenograft

性能

应用

Our Abpromise guarantee covers the use of ab97777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能
    Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
  • 序列相似性
    Belongs to the MAD2 family.
    Contains 1 HORMA domain.
  • 结构域
    The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
  • 翻译后修饰
    Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20.
  • 细胞定位
    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HsMAD 2 antibody
    • HsMAD2 antibody
    • MAD 2 antibody
    • MAD 2A antibody
    • MAD2 like 1 antibody
    • MAD2 like protein 1 antibody
    • MAD2 mitotic arrest deficient like 1 antibody
    • MAD2-like protein 1 antibody
    • Mad2l1 antibody
    • MD2L1_HUMAN antibody
    • Mitotic arrest deficient 2-like protein 1 antibody
    • Mitotic arrest deficient yeast homolog like 1 antibody
    • Mitotic spindle assembly checkpoint protein MAD2A antibody
    see all

Anti-Mad2L1 antibody 图像

  • All lanes : Anti-Mad2L1 antibody (ab97777) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 24 kDa
  • Immunofluorescence analysis of Mad2L1 in Human osteosarcoma cell line U2OS, using ab97777 at 1/1000 dilution.
    1: Mad2L1
    2: ACA
    3: merged with DAPI
    Cells were fixed with 4% PFA/PBS for 15min, RT and blocked with 2.5% BSA/PBS.
  • Immunohistochemical analysis of Mad2L1 in paraffin embedded DLD-1 xenograft, using ab97777 at a 1/500 dilution.

Anti-Mad2L1 antibody (ab97777)参考文献

ab97777 has not yet been referenced specifically in any publications.

Product Wall

You are very welcome. If you have any further questions please to not hesitate to contact us.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册