Anti-Mad2L1抗体[17D10] (ab10691)

概述

  • 产品名称Anti-Mad2L1抗体[17D10]
    参阅全部 Mad2L1 一抗
  • 描述
    小鼠单克隆抗体[17D10] to Mad2L1
  • 特异性Western blot with a variety of human cell lines confirms that the antibody is specific for Mad2.
  • 经测试应用适用于: WB, IP, IHC-Pmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human Mad2.

性能

应用

Our Abpromise guarantee covers the use of ab10691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24.6 kDa).
IP Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
  • 应用说明Is unsuitable for ICC/IF.
  • 靶标

    • 功能Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
    • 序列相似性Belongs to the MAD2 family.
      Contains 1 HORMA domain.
    • 结构域The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
    • 翻译后修饰Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20.
    • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HsMAD 2 antibody
      • HsMAD2 antibody
      • MAD 2 antibody
      • MAD 2A antibody
      • MAD2 like 1 antibody
      • MAD2 like protein 1 antibody
      • MAD2 mitotic arrest deficient like 1 antibody
      • MAD2-like protein 1 antibody
      • Mad2l1 antibody
      • MD2L1_HUMAN antibody
      • Mitotic arrest deficient 2-like protein 1 antibody
      • Mitotic arrest deficient yeast homolog like 1 antibody
      • Mitotic spindle assembly checkpoint protein MAD2A antibody
      see all

    Anti-Mad2L1 antibody [17D10] 图像

    • ab10691 staining Mad2L1 in colon cancer tissue, after antigen retreival, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)



    • Predicted band size : 24.6 kDa

      Western blot using ab10691 at 1/500.

      Lane 1: Hela Nuclear
      Lane 2: Hela
      Lane 3: A431
      Lane 4: Jurkat
      Lane 5: 293

      Mad2L1 predicted size: 24.6kD.

      Secondary ab: Rabbit polyclonal to Mouse IgG H&L (HRP) ab6728 (1/5000)
      Exposure time: 1min
      20µg of lysate per lane.

      Western blot using ab10691 at 1/500. Lane 1: Hela Nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293 Mad2L1 predicted size: 24.6kD. Secondary ab: anti-mouse HRP (ab6728) (1/5000) Exposure time: 1min 20µg of lysate per lane.



    • Predicted band size : 24.6 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)

      This image is courtesy of an Abreview submitted by Saira Sarfraz

      Mad2L1 antibody (ab10691) at 1/1000 (in TBS for 90 minutes at 22°C) + whole tissue lysate of Human liver biopsy (chronic hepatitis C - (HepC) or non-viral (NVH)) patients at 50µg.

      Secondary Antibody: An HRP-conjugated Goat anti-mouse polyclonal (1/100000).

      Blocking Step: 5% Milk for 16 hours at 4°C

      See Abreview

    Anti-Mad2L1 antibody [17D10] (ab10691)参考文献

    This product has been referenced in:
    • Gysin S  et al. Analysis of mRNA profiles after MEK1/2 inhibition in human pancreatic cancer cell lines reveals pathways involved in drug sensitivity. Mol Cancer Res 10:1607-19 (2012). WB ; Human . Read more (PubMed: 22833572) »
    • Sarfraz S  et al. Modulations of cell cycle checkpoints during HCV associated disease. BMC Infect Dis 9:125 (2009). WB ; Human . Read more (PubMed: 19664251) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (Human liver biopsy)
    Loading amount 50 µg
    Specification Human liver biopsy
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dr. Saira Khalid

    Verified customer

    提交于 Sep 03 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Dec 18 2006

    The epitope recognized by this antibody has not been mapped.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"